PXD042023

PXD042023 is an original dataset announced via ProteomeXchange.

Title	Betacoronavirus Internal protein as a determinant of pathogenicity
Description	Human betacoronaviruses encode an Internal (I) protein via an alternative reading frame within the nucleocapsid (N) gene, namely ORF8b for Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) and ORF9b for Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and SARS-CoV-2. Previous reports suggested that ORF8b and ORF9b are involved in evading innate immune responses, but their roles in mediating pathogenesis in infected animals has not been determined. In this study, we abrogated the expression of ORF8b in MERS-CoV (rMERS-Δ8b) and ORF9b in SARS-CoV-2 (rSARS2-Δ9b) and found that rMERS-Δ8b was more virulent while rSARS2-Δ9b was attenuated compared to their respective wild-type (WT) viruses in mice. Upon further analysis, we detected high levels of IFN-I expression and enhanced infiltration of immune cells to the lungs of rMERS-Δ8b-infected mice. In addition, mutations identified in previously circulating SARS-CoV-2 variant of concern (VOC) that were proposed to enhance ORF9b expression did not lead to significant changes in virulence. These data suggest the disparate and virus-specific functions of ORF8b and ORF9b which potentially explain the different changes in virulence.
HostingRepository	PRIDE
AnnounceDate	2023-10-09
AnnouncementXML	Submission_2023-10-09_14:21:11.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EmmaLuhmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-05 09:24:09	ID requested
⏵ 1	2023-10-09 14:21:12	announced

Dataset with its publication pending

submitter keyword: Human, SARS-CoV-2, LC-MS/MS, timsTOF Pro

Dr.Lilliana
contact affiliation	Department of Microbiology and Immunology, University of Iowa, Iowa City, IA
contact email	lilliana-radoshevich@uiowa.edu
lab head
EmmaLuhmann
contact affiliation	University of Iowa
contact email	emma-luhmann@uiowa.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042023

PRIDE project URI

• PRIDE
  1. PXD042023
     1. Label: PRIDE project
     2. Name: Betacoronavirus Internal protein as a determinant of pathogenicity