PXD042014

PXD042014 is an original dataset announced via ProteomeXchange.

Title	TurboID-based proxisome mapping of the human glucocorticoid receptor in A549 cells
Description	Exogenous glucocorticoids are frequently used to treat inflammatory disorders and as adjuncts in solid cancers. However, their use is associated with severe side effects and therapy resistance. Novel glucocorticoid receptor (GR) ligands with a patient-validated reduced side effect profile have not yet reached the clinic. GR is a member of the nuclear receptor family of transcription factors and heavily relies on interactions with coregulator proteins for its transcriptional activity. To elucidate the role of the GR interactome in the altered transcriptional activity of GR following treatment with agonists, antagonists or lead selective GR agonists and modulators (SEGRAMs), we generated comprehensive interactome maps by high-confidence proximity proteomics in lung epithelial carcinoma cells. We found that the GR antagonist RU486 and the SEGRAM Dagrocorat both reduced GR interaction with CREB-binding protein (CBP)/p300 and the mediator complex compared to the full agonist Dexamethasone. Our data offer new insights into the role differential coregulator protein recruitment in shaping specific GR-mediated transcriptional responses.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_03:00:47.261.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042014
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	An Staes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-05 04:19:09	ID requested
⏵ 1	2024-05-24 03:00:48	announced

Van Moortel L, Verhee A, Thommis J, Houtman R, Melchers D, Delhaye L, Van Leene C, Hellemans M, Gevaert K, Eyckerman S, De Bosscher K, Selective Modulation of the Human Glucocorticoid Receptor Compromises GR Chromatin Occupancy and Recruitment of p300/CBP and the Mediator Complex. Mol Cell Proteomics, 23(3):100741(2024) [pubmed]

10.6019/PXD042014;

10.1016/j.mcpro.2024.100741;

submitter keyword: LC-MSMS, TurboID,Glucocorticoid receptor, interactomics

Karolien De Bosscher
contact affiliation	1 VIB-UGent Center for Medical Biotechnology, B9052 Ghent, Belgium; 2 Department of Biomolecular Medicine, Ghent University, B9052 Ghent, Belgium.
contact email	karolien.debosscher@vib-ugent.be
lab head
An Staes
contact affiliation	Medical Protein Chemistry
contact email	an.staes@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042014

PRIDE project URI

• PRIDE
  1. PXD042014
     1. Label: PRIDE project
     2. Name: TurboID-based proxisome mapping of the human glucocorticoid receptor in A549 cells