PXD041995

PXD041995 is an original dataset announced via ProteomeXchange.

Title	ENOD93 interacts with cytochrome c oxidase altering respiratory ATP production and root growth in plants – targeted analysis of peptides for mitochondrial proteins
Description	The early nodulin 93 (ENOD93) gene family in plants can regulate biological nitrogen fixation in legumes and nitrogen use efficiency in cereals but its molecular function is unknown. We show profile hidden Markov models define ENOD93 as a distant homolog of the N-terminal domain of RESPIRATORY SUPERCOMPLEX FACTOR 2 (RCF2). RCF2 is reported to regulate cytochrome oxidase (CIV) influencing the generation of a mitochondria proton motive force in yeast. Knockout of enod93 in Arabidopsis leads to a short root phenotype. ENOD93 is associated with a protein complex the size of CIV in isolated mitochondria but neither CIV abundance nor its activity in ruptured organelles changed in enod93. However, a progressive loss of ADP-dependent respiration rate was observed in enod93 mitochondria which could be fully recovered in complemented lines. Mitochondrial membrane potential was higher in enod93 but ATP synthesis and ADP depletion rates progressively decreased. Respiration rate of whole enod93 seedlings was elevated and root ADP content was nearly double that in WT without a change in ATP content. These altered energetic states correlated with elevated respiratory substrate levels in roots of enod93 compared to WT and complemented lines. Overexpression of ENOD93 lowered ATP content in roots and increased the abundance of a range of amino acids in both roots and leaves. We propose that two previously unconnected gene families in plants, ENOD93 and HYPOXIA INDUCED GENE DOMAIN, are the functional equivalent of yeast RCF2 but have remained undiscovered in many eukaryotic lineages because they are encoded in two separate genes.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:27:32.991.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elke Stroeher
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-04 16:30:26	ID requested
⏵ 1	2025-05-06 09:27:33	announced

10.1093/plcell/koae242;

Lee CP, Le XH, Gawryluk RMR, Casaretto JA, Rothstein SJ, Millar AH, EARLY NODULIN93 acts via cytochrome c oxidase to alter respiratory ATP production and root growth in plants. Plant Cell, 36(11):4716-4731(2024) [pubmed]

submitter keyword: Early nodulin 93

mitochondrial oxidative phosphorylation

RCF2

Complex IV

ATP synthesis

A. Harvey Millar
contact affiliation	School of Molecular Sciences, The University of Western Australia, Perth, WA, Australia. harvey.millar@uwa.edu.au. The ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Perth, WA, Australia. harvey.millar@uwa.edu.au.
contact email	harvey.millar@uwa.edu.au
lab head
Elke Stroeher
contact affiliation	The University of Western Australia
contact email	elke.stroeher@uwa.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041995
     1. Label: PRIDE project
     2. Name: ENOD93 interacts with cytochrome c oxidase altering respiratory ATP production and root growth in plants – targeted analysis of peptides for mitochondrial proteins