PXD041995 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ENOD93 interacts with cytochrome c oxidase altering respiratory ATP production and root growth in plants – targeted analysis of peptides for mitochondrial proteins |
Description | The early nodulin 93 (ENOD93) gene family in plants can regulate biological nitrogen fixation in legumes and nitrogen use efficiency in cereals but its molecular function is unknown. We show profile hidden Markov models define ENOD93 as a distant homolog of the N-terminal domain of RESPIRATORY SUPERCOMPLEX FACTOR 2 (RCF2). RCF2 is reported to regulate cytochrome oxidase (CIV) influencing the generation of a mitochondria proton motive force in yeast. Knockout of enod93 in Arabidopsis leads to a short root phenotype. ENOD93 is associated with a protein complex the size of CIV in isolated mitochondria but neither CIV abundance nor its activity in ruptured organelles changed in enod93. However, a progressive loss of ADP-dependent respiration rate was observed in enod93 mitochondria which could be fully recovered in complemented lines. Mitochondrial membrane potential was higher in enod93 but ATP synthesis and ADP depletion rates progressively decreased. Respiration rate of whole enod93 seedlings was elevated and root ADP content was nearly double that in WT without a change in ATP content. These altered energetic states correlated with elevated respiratory substrate levels in roots of enod93 compared to WT and complemented lines. Overexpression of ENOD93 lowered ATP content in roots and increased the abundance of a range of amino acids in both roots and leaves. We propose that two previously unconnected gene families in plants, ENOD93 and HYPOXIA INDUCED GENE DOMAIN, are the functional equivalent of yeast RCF2 but have remained undiscovered in many eukaryotic lineages because they are encoded in two separate genes. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_09:27:32.991.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elke Stroeher |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monohydroxylated residue |
Instrument | TSQ Altis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-04 16:30:26 | ID requested | |
⏵ 1 | 2025-05-06 09:27:33 | announced | |
Publication List
10.1093/plcell/koae242; |
Lee CP, Le XH, Gawryluk RMR, Casaretto JA, Rothstein SJ, Millar AH, EARLY NODULIN93 acts via cytochrome c oxidase to alter respiratory ATP production and root growth in plants. Plant Cell, 36(11):4716-4731(2024) [pubmed] |
Keyword List
submitter keyword: Early nodulin 93 |
mitochondrial oxidative phosphorylation |
RCF2 |
Complex IV |
ATP synthesis |
Contact List
A. Harvey Millar |
contact affiliation | School of Molecular Sciences, The University of Western Australia, Perth, WA, Australia. harvey.millar@uwa.edu.au. The ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Perth, WA, Australia. harvey.millar@uwa.edu.au. |
contact email | harvey.millar@uwa.edu.au |
lab head | |
Elke Stroeher |
contact affiliation | The University of Western Australia |
contact email | elke.stroeher@uwa.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041995
- Label: PRIDE project
- Name: ENOD93 interacts with cytochrome c oxidase altering respiratory ATP production and root growth in plants – targeted analysis of peptides for mitochondrial proteins