PXD041966

PXD041966 is an original dataset announced via ProteomeXchange.

Title	Pick-up single-cell proteomic analysis for quantifying up to 3000 proteins in a tumor cell
Description	The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ~1000 proteins per cell is still insufficient for practical applications. Here, we develop a pick-up single-cell proteomic analysis (PiSPA) workflow to achieve a deep identification capable of quantifying up to 3000 protein groups in a tumor cell using the label-free quantitative method. The PiSPA workflow is specially established for single-cell samples mainly based on a nanoliter-scale microfluidic liquid handling robot, capable of achieving single-cell capture, pretreatment and injection under the pick-up operation strategy. Using this customized workflow with remarkable improvement in protein identification, 1804–3349, 1778–3049 and 1074–2487 protein groups are quantified in single A549 cells (n = 37), HeLa cells (n = 44) and U2OS cells (n = 27), respectively. Benefiting from the flexible cell picking-up ability, we study tumor cell migration at the single cell proteome level, demonstrating the potential in practical biological research from single-cell insight.
HostingRepository	iProX
AnnounceDate	2023-10-30
AnnouncementXML	Submission_2024-02-12_04:36:15.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-03 20:59:29	ID requested
⏵ 1	2024-02-12 04:36:16	announced

Wang Y, Guan ZY, Shi SW, Jiang YR, Zhang J, Yang Y, Wu Q, Wu J, Chen JB, Ying WX, Xu QQ, Fan QX, Wang HF, Zhou L, Wang L, Fang J, Pan JZ, Fang Q, Pick-up single-cell proteomic analysis for quantifying up to 3000 proteins in a Mammalian cell. Nat Commun, 15(1):1279(2024) [pubmed]

submitter keyword: single-cell proteomics, microfluidics, cell migration, shotgun proteomics

Qun Fang
contact affiliation	Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University
contact email	fangqun@zju.edu.cn
lab head
Yu Wang
contact affiliation	Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University
contact email	wangyu310@zju.edu.cn
dataset submitter

iProX dataset URI