PXD041943

PXD041943 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of the GPER1 interactome
Description	The G-protein coupled estrogen receptor GPER1 is thought to mediate rapid estrogen signaling at the membrane. Despite considerable efforts over the last years, its physiological ligand(s), interactors, and regulators are still very poorly understood. This is particularly problematic at a time when the inducibility of GPER1 activity has been challenged. We therefore investigated both activity and interactome of GPER1. We could confirm that GPER1 can behave as a constitutivley active signaling protein. Using proximity labelling and immunoprecipitation coupled to mass spectrometry, we explored the GPER1 interactome. Intersecting the results of the two proteomic approaches and validating some candidate interactors, we generate a GPER1 a resource, which may be useful for further functional studies in the future.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:39.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-03 03:04:53	ID requested
1	2023-11-06 02:22:14	announced
2	2023-11-14 08:24:12	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:40	announced	2024-10-22: Updated project metadata.

10.3390/CELLS12212571;

submitter keyword: G-protein coupled estrogen receptor

membrane protein

estrogen signaling

protein-protein interactions

proximity labeling

Didier Picard
contact affiliation	Département de Biologie Moléculaire et Cellulaire, Université de Genève
contact email	didier.picard@unige.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041943
     1. Label: PRIDE project
     2. Name: Proteomic identification of the GPER1 interactome