PXD041918

PXD041918 is an original dataset announced via ProteomeXchange.

Title	The proteome of extracellular vesicles from Haemonchus contortus
Description	Parasitic worms have a major global impact in human and animal populations due to the chronicity of their infections. These parasites have an exquisite capacity to survive in host animals. There is a growing body of evidence that extracellular vesicles (EVs) are intimately involved in parasitism, and that these vesicles are instrumental in modulating (suppressing) inflammatory/immune responses. The recent expansion of proteomic and lipidomic technologies and molecular resources for some parasitic worms (order Strongylida) provide a unique opportunity to conduct profound (qualitative and quantitative) explorations of EVs, with the prospect of being able to elucidate the functional roles of key molecules within these vesicles in host tissues and cells. As one of the most pathogenic nematodes of livestock animals, the blood-feeding barber’s pole worm – Haemonchus contortus – is an ideal model system for EV exploration. Here, using advanced methods, we defined the proteome of EVs of Haemonchus contortus. We identified and quantified 561 proteins in these EVs, and compared these molecules with those of adult worms. We identified unique molecules in EVs such as proteins linked to lipid transportation and lipid species (i.e. glycerophospholipids and sphingolipids) associated with signalling, indicating an involvement of these molecules in parasite-host cross-talk. This work provides a solid starting point to explore the functional roles of EV-specific proteins and lipids in modulating parasite-host cross talk; a foundation to assess the utility of such molecules as biomarkers for the specific detection of infection or disease; and the prospect of finding ways to disrupt or interrupt this relationship to suppress or eliminate infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:53.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-01 21:15:40	ID requested
1	2023-08-06 16:29:15	announced
2	2023-11-14 09:04:29	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:59:54	announced	2024-10-22: Updated project metadata.

Wang T, Koukoulis TF, Vella LJ, Su H, Purnianto A, Nie S, Ang CS, Ma G, Korhonen PK, Taki AC, Williamson NA, Reid GE, Gasser RB, to Underpin Explorations of Host-Parasite Cross-Talk. Int J Mol Sci, 24(13):(2023) [pubmed]

10.3390/ijms241310955;

submitter keyword: Haemonchus contortus, proteomics, LC-MS/MS

Robin Beat Gasser
contact affiliation	Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD041918

PRIDE project URI

• PRIDE
  1. PXD041918
     1. Label: PRIDE project
     2. Name: The proteome of extracellular vesicles from Haemonchus contortus