PXD041917 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth |
Description | This study aimed to use a lamb model of preterm birth to investigate the impact of different tidal volume strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 15 minutes, followed by a 30 minute ‘rest’ period where lambs were supported via the intact placenta. A group of lambs was also euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Q Exactive HF-X benchtop Orbitrap mass spectrometer in a data-dependent acquisition mode. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:05:20.942.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | david greening |
SpeciesList | scientific name: Ovis aries; NCBI TaxID: 9940; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-01 20:33:05 | ID requested | |
1 | 2023-10-09 08:15:44 | announced | |
2 | 2023-11-14 09:05:52 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:05:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
Pereira-Fantini PM, Kenna KR, Fatmous M, Sett A, Douglas E, Dahm S, Sourial M, Fang H, Greening DW, Tingay DG, Impact of tidal volume strategy at birth on initiating lung injury in preterm lambs. Am J Physiol Lung Cell Mol Physiol, 325(5):L594-L603(2023) [pubmed] |
10.1152/ajplung.00159.2023; |
Keyword List
submitter keyword: ventilation,proteomics, TMT, lung, tissue damage, sheep |
Contact List
David Greening & Prudence Pereira-Fantini |
contact affiliation | Baker Heart & Diabetes Institute and Murdoch Childrens Research Institute (pmpf@unimelb.edu.au) |
contact email | david.greening@baker.edu.au |
lab head | |
david greening |
contact affiliation | Baker Heart and Diabetes Inst. |
contact email | david.greening@baker.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041917
- Label: PRIDE project
- Name: Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth