PXD041917

PXD041917 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth
Description	This study aimed to use a lamb model of preterm birth to investigate the impact of different tidal volume strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 15 minutes, followed by a 30 minute ‘rest’ period where lambs were supported via the intact placenta. A group of lambs was also euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Q Exactive HF-X benchtop Orbitrap mass spectrometer in a data-dependent acquisition mode.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:20.942.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	david greening
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-01 20:33:05	ID requested
1	2023-10-09 08:15:44	announced
2	2023-11-14 09:05:52	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:05:22	announced	2024-10-22: Updated project metadata.

Pereira-Fantini PM, Kenna KR, Fatmous M, Sett A, Douglas E, Dahm S, Sourial M, Fang H, Greening DW, Tingay DG, Impact of tidal volume strategy at birth on initiating lung injury in preterm lambs. Am J Physiol Lung Cell Mol Physiol, 325(5):L594-L603(2023) [pubmed]

10.1152/ajplung.00159.2023;

submitter keyword: ventilation,proteomics, TMT, lung, tissue damage, sheep

David Greening & Prudence Pereira-Fantini
contact affiliation	Baker Heart & Diabetes Institute and Murdoch Childrens Research Institute (pmpf@unimelb.edu.au)
contact email	david.greening@baker.edu.au
lab head
david greening
contact affiliation	Baker Heart and Diabetes Inst.
contact email	david.greening@baker.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD041917

PRIDE project URI

• PRIDE
  1. PXD041917
     1. Label: PRIDE project
     2. Name: Proteomics analysis of preterm lung tissue with mass spectrometry to understand the impact of tidal volume strategy at birth