PXD041915
PXD041915 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics of SEC and IEX fractionated Mouse Embryonic Stem Cells |
| Description | Mouse embryonic stem cells were lysed in Pierce IP Lysis buffer and 2 mg of the clarified extract was fractionated on an HPLC SEC column and 2 mg was fractionated on an HPLC IEX mixed bed column. All fractions were reduced, alkylated, and trypsin digested for mass spectrometry as in Mallam et al. 2019 (PMID: 31665645). Data were collected on an Orbitrap Fusion mass spectrometer using a data dependent 75 minute topspeed method. Dissociation for SEC fractions was by CID, and for IEX fractions was by HCD. RAW files were converted to mzXML and searched using an MSBlender analysis pipeline. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-06 |
| AnnouncementXML | Submission_2024-03-06_11:19:43.479.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ophelia Papoulas |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-05-01 15:20:24 | ID requested | |
| ⏵ 1 | 2024-03-06 11:19:43 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: LECA, MES, mouse embryonic stem cell, SEC, IEX, orbitrap fusion |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas-Austin |
| contact email | marcotte@icmb.utexas.edu |
| lab head | |
| Ophelia Papoulas | |
| contact affiliation | University of Texas at Austin |
| contact email | papoulas@austin.utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091831/ |
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