PXD041878

PXD041878 is an original dataset announced via ProteomeXchange.

Title	Protein interaction network revealed by quantitative proteomic analysis elucidates TFIIB role in multiple aspects of the transcription cycle
Description	Although TFIIB is widely regarded as an initiation factor, recent reports have implicated it in multiple aspects of eukaryotic transcription. To investigate the broader role of TFIIB in transcription, we performed quantitative proteomic analysis of yeast TFIIB. We purified two different populations of TFIIB; one form soluble cell lysate, which is not engaged in transcription, and the other from chromatin fraction which yields the transcriptionally active form of the protein. TFIIB purified from the chromatin exhibits several interactions that explain its non-canonical roles in transcription. RNAPII, TFIIF and TFIIH were the only components of preinitiation complex with a significant presence in chromatin TFIIB. A notable feature was the substantial enrichment of all 3’ end processing-termination factors; CF1, CPF and Rat1 in chromatin-TFIIB preparation. These results corroborate the role of TFIIB in termination of transcription. Presence of the Lsm complex as well as TREX complex subunit Sub2 in chromatin-TFIIB opens the possibility of novel roles of TFIIB in synthesis-decay coupling and nucleocytoplasmic transport of mRNA. This multiplicity of functions may contribute to the preferential targeting of TFIIB during viral pathogenesis
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:32.989.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041878
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Stemmer
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-28 10:14:00	ID requested
1	2024-05-24 02:21:53	announced
⏵ 2	2024-10-22 06:42:38	announced	2024-10-22: Updated project metadata.

10.1016/j.bbapap.2023.140968;

O'Brien MJ, Ansari A, Protein interaction network revealed by quantitative proteomic analysis links TFIIB to multiple aspects of the transcription cycle. Biochim Biophys Acta Proteins Proteom, 1872(1):140968(2024) [pubmed]

10.6019/PXD041878;

submitter keyword: Budding Yeast, Proteomic Analyses, RNA polymerase II, TFIIB, Transcription

Athar Ansari
contact affiliation	Department of Biological Sciences Wayne state University
contact email	bb2749@wayne.edu
lab head
Paul Stemmer
contact affiliation	Wayne State University
contact email	pmstemmer@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041878
     1. Label: PRIDE project
     2. Name: Protein interaction network revealed by quantitative proteomic analysis elucidates TFIIB role in multiple aspects of the transcription cycle