PXD041861

PXD041861 is an original dataset announced via ProteomeXchange.

Title	Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells
Description	This study searched for cell-type specific effectors of endocytic escape in dendritic cells and identified a pore-forming protein, perforin-2 (encoded by Mpeg1), as a dedicated effector exclusive to cross-presenting cells. Whole cell proteomics identified differences in the abundance of tryptic and semi-tryptic (cleaved) perforin-2 peptides between control MutuDCs and cells treated with BafA, CpG or with knockout of asparagine endopeptidase (AEP). This revealed how perforin-2 undergoes proteolytic cleavage releasing its pore forming domain into the organellar lumen.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:54:25.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandra Davies
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-27 16:18:08	ID requested
1	2023-06-27 02:53:00	announced
⏵ 2	2023-11-14 06:54:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Dendritic cells, antigen, cross-presentation, perforin-2

Georg H. H. Borner
contact affiliation	Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany
contact email	borner@biochem.mpg.de
lab head
Alexandra Davies
contact affiliation	University of Manchester
contact email	alexandra.davies-2@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041861

PRIDE project URI

• PRIDE
  1. PXD041861
     1. Label: PRIDE project
     2. Name: Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells