PXD041848

PXD041848 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Complexome Profiling of the chloroplast proteome from Arabidopsis thaliana
Description	Chloroplasts are the metabolically most active compartment of mature leaf cells. Their proteins are involved in essential cellular processes, such as photosynthesis, nitrogen fixation, and fatty acid synthesis. For this, chloroplast proteins have to associate with other fellow organellar proteins to form functional units, react to changing environmental conditions, or optimize efficiency of biochemical reactions. We here investigated chloroplast protein-protein interactions by a combination of complexome profiling and cross-linking mass spectrometry (CX-MS). Different detergents and MS settings were tested for developing a new workflow, which was found to produce data of improved quality when comparted to standard complexome profiling approaches. This procedure was applied to chloroplasts acclimated to increasing light intensities to investigate the role of protein-protein interactions in the adaption to these conditions.
HostingRepository	MassIVE
AnnounceDate	2024-07-02
AnnouncementXML	Submission_2024-07-02_04:24:22.510.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noah Ditz
SpeciesList	scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-27 06:08:39	ID requested
⏵ 1	2024-07-02 04:24:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: LC-MSMS, Blue Native, proteome, chloroplast, Arabidopsis thaliana, complexome profiling, cross-linking

submitter keyword: LC-MSMS, Blue Native, proteome, chloroplast, Arabidopsis thaliana, complexome profiling, cross-linking

Contact List

Hans-Peter Braun
contact affiliation	Plant Genetics, department plant proteomics
contact email	braun@genetik.uni-hannover.de
lab head
Noah Ditz
contact affiliation	Leibniz University Hannover
contact email	ditz@genetik.uni-hannover.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091808/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091808/