PXD041847

PXD041847 is an original dataset announced via ProteomeXchange.

Title	A single chlamydial protein reshapes the plasma membrane and serves as recruiting platform for central endocytic effector proteins
Description	Uptake of obligate intracellular bacterial pathogens into mammalian epithelial cells is critically dependent on modulation of the host’s endocytic machinery. It is an open question how the invading pathogens generate a membrane-bound vesicle appropriate to their size. This requires extensive deformation of the host plasma membrane itself by pathogen-derived membrane-binding proteins, accompanied by substantial F-actin-based forces to further expand and finally pinch off the vesicle. Here we show that upon adhesion to the host cell, the human pathogenic bacterium Chlamydia pneumoniae secretes the scaffolding effector protein CPn0677, which binds to the inner leaflet of the invaginating host’s PM, induces inwardly directed, negative membrane curvature, and forms a recruiting platform for the membrane-deforming BAR-domain containing proteins Pacsin and SNX9. In addition, while bound to the membrane, CPn0677 recruits monomeric G-actin, and its C-terminal region binds and activates N-WASP, which initiates branching actin polymerization via the Arp2/3 complex. Together, these membrane-bound processes enable the developing endocytic vesicle to engulf the infectious elementary body, while the associated actin network generates the forces required to reshape and detach the nascent vesicle from the PM. Thus, Cpn0677 (now renamed SemD) acts as recruiting platform for central components of the endocytic machinery during uptake of chlamydia.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:05.241.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Chlamydia pneumoniae; NCBI TaxID: 83558; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-27 05:41:36	ID requested
1	2023-05-15 00:50:45	announced
⏵ 2	2023-11-14 08:48:06	announced	2023-11-14: Updated project metadata.

Spona D, Hanisch PT, Hegemann JH, M, ö, lleken K, A single chlamydial protein reshapes the plasma membrane and serves as recruiting platform for central endocytic effector proteins. Commun Biol, 6(1):520(2023) [pubmed]

submitter keyword: epithelial cell, actin binding, Chlamydia pneumoniae, SemD,pathogen, infection, endocytosis, membrane

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine Heinrich Heine University Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041847
     1. Label: PRIDE project
     2. Name: A single chlamydial protein reshapes the plasma membrane and serves as recruiting platform for central endocytic effector proteins