PXD041819

PXD041819 is an original dataset announced via ProteomeXchange.

Title	Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion and Migration in THP-1 Cells
Description	The interaction between monocytes and endothelial cells in inflammation is central to chemoattraction, adhesion, and transendothelial migration. Key players such as selectins and their ligands, integrins and other adhesion molecules and their function in these processes are well studied. Toll-like receptor 2 (TLR2), expressed on monocytes, is critical for sensing invading pathogens and initiating a rapid and effective immune response. However, the extended role of TLR2 in monocyte adhesion and migration has only been partially elucidated. To address this question, we performed several functional cell-based assays using monocyte-like wild type (WT), TLR2-knock-out (KO) and, TLR2-knock-in (KI) THP-1 cells. We found that TLR2 promotes faster and stronger adhesion of monocytes to the endothelium and a more intense endothelial barrier disruption after endothelial activation. In addition, we performed quantitative mass-spectrometry, STRING protein analysis, and RT-qPCR, which revealed the association of TLR2 with specific integrins, but also uncovered novel proteins affected by TLR2. In conclusion, we could show that unstimulated TLR2 affects cell adhesion, endothelial barrier disruption, migration, and actin polymerization.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:38.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Neuditschko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-26 01:44:00	ID requested
1	2023-10-24 12:40:53	announced
⏵ 2	2023-11-14 09:09:47	announced	2023-11-14: Updated project metadata.

Colleselli K, Ebeyer-Masotta M, Neuditschko B, Stierschneider A, Pollhammer C, Potocnjak M, Hundsberger H, Herzog F, Wiesner C, Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion, and Migration in THP-1 Cells. Cells, 12(10):(2023) [pubmed]

submitter keyword: cell adhesion

cell migration,Toll-like receptor 2

chemotaxis, THP-1

Franz Herzog
contact affiliation	Institute Krems Bioanalytics, IMC University of Applied Sciences, 3500 Krems, Austria
contact email	franz.herzog@fh-krems.ac.at
lab head
Benjamin Neuditschko
contact affiliation	Institute Krems Bioanalytics, IMC University of Applied Sciences Krems, 3500 Krems, Austria
contact email	benjamin.neuditschko@fh-krems.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD041819

PRIDE project URI

• PRIDE
  1. PXD041819
     1. Label: PRIDE project
     2. Name: Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion and Migration in THP-1 Cells