PXD041799

PXD041799 is an original dataset announced via ProteomeXchange.

Title	Haloferax volcanii lysine acetylation
Description	Oxidative stress adaptation strategies are important to cell function and are linked to cardiac, neurodegenerative disease and cancer. Representatives of the domain Archaea are used as model organisms based on their extreme tolerance to oxidants and close evolutionary relationship with eukaryotes. Study of the halophilic archaeon Haloferax volcanii reveals lysine acetylation to be associated with oxidative stress responses. The strong oxidant hypochlorite: i) stimulates an increase in lysine acetyltransferase HvPat2 to HvPat1 abundance ratios and ii) selects for lysine deacetylase sir2 mutants. Here we report the dynamic occupancy of the lysine acetylome of glycerol-grown H. volcanii as it shifts in profile in response to hypochlorite. These findings are revealed by the: 1) quantitative multiplex LC-MS/MS analysis of the SILAC-compatible parent and Δsir2 mutant strains and 2) label free LC-MS/MS analysis of H26 ‘wild type’ cells. The results show that lysine acetylation is associated with key biological processes including DNA topology, central metabolism, cobalamin biosynthesis and translation. Lysine acetylation targets are found conserved across species. Moreover, lysine residues modified by acetylation and ubiquitin-like sampylation are identified suggesting post-translational modification (PTM) crosstalk. Overall, the results of this study expand the current knowledge of lysine acetylation in Archaea, with the long-term goal to provide a balanced evolutionary perspective of PTM systems in living organisms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:56:49.777.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ricardo Couto-Rodriguez
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-25 09:05:24	ID requested
1	2023-07-20 12:08:35	announced
2	2023-11-14 09:03:53	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:56:51	announced	2024-10-22: Updated project metadata.

10.3390/antiox12061203;

Couto-Rodr, í, guez RL, Koh J, Chen S, Maupin-Furlow JA, during Oxidative Stress by Quantitative SILAC-Based Proteomics. Antioxidants (Basel), 12(6):(2023) [pubmed]

submitter keyword: PTMs, halophiles, acetylation,Haloferax volcanii, oxidative stress

Julie Maupin-Furlow
contact affiliation	Microbiology and Cell Science at University of Florida
contact email	jmaupin@ufl.edu
lab head
Ricardo Couto-Rodriguez
contact affiliation	University of Florida
contact email	r.coutorodriguez@ufl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041799
     1. Label: PRIDE project
     2. Name: Haloferax volcanii lysine acetylation