PXD041791

PXD041791 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of extracellular membrane vesicles from Streptococcus sanguinis
Description	Streptococcus sanguinis, a Gram-positive oral commensal, is highly prevalent in the oral cavity and well-characterized for its ability to antagonize oral pathobionts. We have found that production and dissemination of membrane vesicles by S. sanguinis is dependent on environmental and community factors. Co-culture with interacting commensal Corynnyebacterium durum, as well as with the periodontal pathobiont Filifactor alocis had no effect on S. sanguinis vesicle number and size, whereas the periodontal pathobiont Porphyromonas gingivalis abolished S. sanguinis vesicle production. Using both correlation and differential expression analyses to examine the transcriptomic changes underlying vesicle production, we found that differential expression of genes encoding proteins related to the cytoplasmic membrane and peptidoglycan correlate with the abundance of membrane vesicles. Proteomic characterizations of the vesicle cargo identified a variety of proteins, including those predicted to influence host interactions or host immune responses. Cell culture studies of gingival epithelial cells demonstrated that both crude and highly purified membrane vesicles could induce the expression of IL-8, TNF-α, IL-1β, and Gro-α within 6 hours of inoculation at levels comparable to whole cells. OTaken together, our findings suggest that production of membrane vesicles by S. sanguinis is heavily influenced by community and environmental factors, and plays an important role in communication with host cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:38:42.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emily Helliwell
SpeciesList	scientific name: Streptococcus sanguinis SK36; NCBI TaxID: 388919;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 22:25:05	ID requested
⏵ 1	2024-10-17 02:38:42	announced

10.1038/s41396-023-01456-3;

Helliwell E, Choi D, Merritt J, Kreth J, Environmental influences on Streptococcus sanguinis membrane vesicle biogenesis. ISME J, 17(9):1430-1444(2023) [pubmed]

submitter keyword: Streptococcus sanguinis,membrane vesicles, commensalism, oral microbiome

Jens Kreth
contact affiliation	Department of Restorative Dentistry, School of Dentistry, Oregon Health and Science University
contact email	kreth@ohsu.edu
lab head
Emily Helliwell
contact affiliation	Oregon Health and Science University
contact email	helliwel@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD041791

PRIDE project URI

• PRIDE
  1. PXD041791
     1. Label: PRIDE project
     2. Name: Proteomic characterization of extracellular membrane vesicles from Streptococcus sanguinis