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PXD041774

PXD041774 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicry.
DescriptionInsertion of lipopolysaccharide (LPS) into the outer membrane (OM) of Gram-negative bacteria is mediated by a druggable OM translocon consisting of a beta-barrel membrane protein, LptD, and a lipoprotein, LptE. The beta-barrel assembly machinery (BAM) assembles LptD together with LptE to form a plug-and-barrel structure. In the enterobacterium Escherichia coli, formation of two native disulfide bonds in LptD controls LPS translocon activation. Here we report the discovery of LptM (formerly YifL), a conserved lipoprotein that assembles together with LptD and LptE at the BAM complex. We demonstrate that LptM stabilizes a conformation of LptD that can efficiently acquire native disulfide bonds and be released as mature LPS translocon by the BAM complex. Inactivation of LptM causes the accumulation of non-natively oxidized LptD, making disulfide bond isomerization by DsbC become essential for viability. Our structural prediction and biochemical analyses indicate that LptM binds to sites in both LptD and LptE that are proposed to coordinate LPS insertion into the OM. These results suggest that, by mimicking LPS binding, LptM facilitates oxidative maturation of LptD, thereby activating the LPS translocon.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:05:32.113.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulien Marcoux
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-24 06:40:34ID requested
12023-10-11 06:24:03announced
22023-11-14 09:05:11announced2023-11-14: Updated project metadata.
32024-10-22 06:05:32announced2024-10-22: Updated project metadata.
Publication List
10.1038/S41467-023-42007-W;
Keyword List
submitter keyword: LptDE, native MS, HDX-MS, membrane protein, translocon
Contact List
Julien Marcoux
contact affiliationIPBS-Toulouse BP64182 205 route de Narbonne 31077 Toulouse Cedex04
contact emailjulien.marcoux@ipbs.fr
lab head
Julien Marcoux
contact affiliationIPBS
contact emailjulien.marcoux@ipbs.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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