PXD041763

PXD041763 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	HEK293T cells were transfected with Flag-tagged NLRP3 treated with or without nigericin (10 μM, 1 h).
Description	In order to identificate NLRP3 acetylation, we utilized an acetylproteomic approach to identify the acetylation sites within NLRP3. We first reconstituted the NLRP3 inflammasome in HEK293T cells by co-transfected with plasmids encoding NLRP3, ASC, Caspase-1 and Pro-IL-1β, then stimulated the genetically modified cells with nigericin.Flag-tagged NLRP3 was immunoprecipitated and analyzed by liquid chromatography-mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-11-25
AnnouncementXML	Submission_2023-11-24_20:16:51.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kai Zhao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 02:01:13	ID requested
⏵ 1	2023-11-24 20:16:51	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: HEK293Tcells， NLRP3

submitter keyword: HEK293Tcells， NLRP3

Contact List

Kai Zhao
contact affiliation	the Third Xiangya Hospital, Central South University
contact email	kaizhao@csu.edu.cn
lab head
Kai Zhao
contact affiliation	the Third Xiangya Hospital
contact email	kaizhao@csu.edu.cn
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD041763
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD041763

PRIDE project URI

Repository Record List

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