PXD041761

PXD041761 is an original dataset announced via ProteomeXchange.

Title	Xenopus laevis kidney-induced animal caps nanoLC-MS/MS - Xenopus Ssbp2 is required for embryonic pronephros morphogenesis and terminal differentiation.
Description	The nephron, functional unit of the vertebrate kidney, is specialized in metabolic wastes excretion and body fluids osmoregulation. Given the high evolutionary conservation of gene expression and segmentation patterning between mammalian and amphibian nephrons, the Xenopus laevis pronephric kidney offers a simplified model for studying nephrogenesis. The Lhx1 transcription factor plays critical roles in kidney development, regulating target gene expression by forming multiprotein complexes with LIM binding protein 1 (Ldb1). However, few Lhx1-Ldb1 cofactors have been identified for this organ formation. By tandem- affinity purification from kidney-induced Xenopus animal caps, we identified single-stranded DNA binding protein 2 (Ssbp2) to interact with the Ldb1-Lhx1 complex. Ssbp2 is expressed in the Xenopus pronephros, and knockdown prevents normal morphogenesis and differentiation of the glomus and the convoluted renal tubules. We demonstrate a role for a member of the Ssbp family in kidney organogenesis and provide evidence of a fundamental function for the Ldb1-Lhx1-Ssbp transcriptional complexes in embryonic development.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:41.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Cirio
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 01:50:18	ID requested
1	2023-10-24 12:29:29	announced
⏵ 2	2023-11-14 09:09:42	announced	2023-11-14: Updated project metadata.

Cervino AS, Collodel MG, Lopez IA, Roa C, Hochbaum D, Hukriede NA, Cirio MC, Xenopus Ssbp2 is required for embryonic pronephros morphogenesis and terminal differentiation. Sci Rep, 13(1):16671(2023) [pubmed]

submitter keyword: Xenopus, animal caps, lhx1, ssbp2, Kidney

Mairia Cecilia Cirio
contact affiliation	University of Buenos Aires, Argentina, Institute of Physiology, Molecular Biology and Neuroscience (UBA_CONICET)
contact email	mcc19mc@gmail.com
lab head
Maria Cirio
contact affiliation	Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE) and Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina.
contact email	mcc19mc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041761
     1. Label: PRIDE project
     2. Name: Xenopus laevis kidney-induced animal caps nanoLC-MS/MS - Xenopus Ssbp2 is required for embryonic pronephros morphogenesis and terminal differentiation.