PXD041759

PXD041759 is an original dataset announced via ProteomeXchange.

Title	Comparative physiological, biochemical and proteomic analyses reveal key proteins and crucial regulatory pathways related to drought stress tolerance in faba bean (Vicia faba L.) leaves
Description	Drought is one of the most crucial environmental stresses limiting faba bean growth and productivity in the Mediterranean region. In order to explore how the faba bean plant responds to drought stress, a physiological and proteomic analysis was performed in leaf tissue. All physiological parameters were affected by drought. The physiological mechanism underlying the response of faba bean leaves to drought was therefore attributed to the alleviation of oxidative stress via the accumulation of organic solutes such as proline and to the synergistic action of the antioxidant enzyme system (CAT, SOD, APX and GPOX). Proteomic analysis identified 2000 proteins from faba bean leaves, of which were 81 differentially expressed. Of those, 45 were upregulated and 36 were downregulated under drought treatment. GO and KEGG enrichments indicated differentially abundant proteins (DAPs) associated with photosynthesis, antioxidants/detoxifying enzymes, molecular chaperones, biosynthesis of amino acids and secondary metabolites, signal transduction, energy and carbohydrate metabolism and metabolic enzymes. The current results provide evidence for a complex synergetic pathway, in which ROS detoxification mechanisms and photoprotection constituted the major aspect of drought tolerance in faba bean leaves. These results offer a foundational basis regarding the molecular mechanism involved in drought resistance within the faba bean species
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:47.621.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041759
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Vicia faba var. minor; NCBI TaxID: 3907;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 00:59:47	ID requested
1	2024-04-30 00:49:08	announced
⏵ 2	2024-10-22 06:37:48	announced	2024-10-22: Updated project metadata.

10.1016/J.CPB.2024.100320;

10.6019/PXD041759;

submitter keyword: differentially abundant proteins (DAPs)

drought stress

faba bean

mass spectrometry

proteomics

physiological analysis

qRT-PCR

Yordan Muhovski
contact affiliation	Department of Life Sciences, Walloon Agricultural Research Centre, Chaussée de Charleroi, P.B. 234, 5030 Gembloux, Belgium
contact email	y.muhovski@cra.wallonie.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041759
     1. Label: PRIDE project
     2. Name: Comparative physiological, biochemical and proteomic analyses reveal key proteins and crucial regulatory pathways related to drought stress tolerance in faba bean (Vicia faba L.) leaves