PXD041758

PXD041758 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Omics analyses of 3T3-J2 feeder cells to identify culture conditions that support the long-term culture of human pancreatic progenitor cells
Description	Multipotent pancreatic progenitor cells have been successfully cultured, expanded and differentiated into cells of the adult human pancreas. The long-term culture of these progenitors (termed cPP) relies on support from mitotically inactivated 3T3-J2 mouse embryonic fibroblasts. The 3T3-J2-derived signals that support cPP expansion and maintain the fine balance between self-renewal and lineage commitment in this co-culture system remain unknown. To unmask these signals, we exploited a striking disparity in morphology and the capacity to self-renew of cPP cultured on 3T3-J2 cells from different sources. We deployed a comparative approach to identify (1) genes that define expandable cPP through transcriptome analysis and (2) key factors secreted by 3T3-J2 using selective enrichment of newly synthesised proteins and mass spectrometry analysis. Using cPP from three genetic backgrounds cultured on either supportive or non-supportive 3T3-J2, these analyses revealed 100 transcription factor genes that are significantly down-regulated in cPP cultured on non-supportive 3T3-J2. Analysis of differentially expressed 3T3-J2 proteins identified 53 whose expression is higher in supportive 3T3-J2. These results provide significant insight into the requirements not only for optimal cPP culture and expansion but also for progenitor cell types in other organ systems that rely on 3T3-J2 co-culture, most notably keratinocytes for clinical applications.
HostingRepository	jPOST
AnnounceDate	2024-04-24
AnnouncementXML	Submission_2024-04-23_08:00:17.451.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Radoslaw Sobota
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; Label:13C(6)15N(2); Label:13C(6)15N(4); alpha-amino acetylated residue; L-methionine sulfoxide; deamidated L-glutamine; L-citrulline
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-24 00:52:51	ID requested
⏵ 1	2024-04-23 08:00:17	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: 3T3-J2 cells, human pancreatic progenitor cells, keratinocytes

submitter keyword: 3T3-J2 cells, human pancreatic progenitor cells, keratinocytes

Contact List

Norris Ray Dunn
lab head
Radoslaw Sobota
contact affiliation	IMCB A-STAR Singapore
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002100/