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PXD041743

PXD041743 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCo-immunoprecipitation assisted mass spectrometry proteomics study of ZBED3-binding protein
DescriptionNonalcoholic fatty liver disease (NAFLD) has emerged as the most prevalent chronic liver disease worldwide. Triglyceride (TG) accumulation is a key pathological feature of NAFLD. Therefore, the understanding of the molecular mechanisms involved in TG accumulation may lead to the development of potential drug targets for the treatment of NAFLD. In this study, we found that zinc finger BED-type containing 3 (ZBED3) was significantly upregulated in the livers of NAFLD patients and animal models. Here, we found that ZBED3 directly interacts with polypyrimidine bundle binding protein 1 (PTBP1) and affects its binding to sterol regulatory element binding protein 1c (SREBP1c) mRNA precursors, thereby regulating mRNA stability and alternative splicing based on co-immunoprecipitation and mass spectrometry. This result reveals the specific mechanism by which ZBED3 overexpression promotes free fatty acid-induced TG accumulation in hepatocytes.
HostingRepositoryiProX
AnnounceDate2023-04-21
AnnouncementXMLSubmission_2023-04-23_00:40:19.019.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaohe Luo
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-23 00:40:04ID requested
12023-04-23 00:40:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cell, Proteome profiling, ZBED3 overexpression, Co-Immunoprecipitation
Contact List
Xiaohe Luo
contact affiliationDepartment of Laboratory Medicine, Chongqing University Three Gorges Hospital
contact emailxiaoheluo@163.com
lab head
Xiaohe Luo
contact affiliationChongqing University
contact emailxiaoheluo@163.com
dataset submitter
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