PXD041743
PXD041743 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-immunoprecipitation assisted mass spectrometry proteomics study of ZBED3-binding protein |
| Description | Nonalcoholic fatty liver disease (NAFLD) has emerged as the most prevalent chronic liver disease worldwide. Triglyceride (TG) accumulation is a key pathological feature of NAFLD. Therefore, the understanding of the molecular mechanisms involved in TG accumulation may lead to the development of potential drug targets for the treatment of NAFLD. In this study, we found that zinc finger BED-type containing 3 (ZBED3) was significantly upregulated in the livers of NAFLD patients and animal models. Here, we found that ZBED3 directly interacts with polypyrimidine bundle binding protein 1 (PTBP1) and affects its binding to sterol regulatory element binding protein 1c (SREBP1c) mRNA precursors, thereby regulating mRNA stability and alternative splicing based on co-immunoprecipitation and mass spectrometry. This result reveals the specific mechanism by which ZBED3 overexpression promotes free fatty acid-induced TG accumulation in hepatocytes. |
| HostingRepository | iProX |
| AnnounceDate | 2023-04-21 |
| AnnouncementXML | Submission_2023-04-23_00:40:19.019.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaohe Luo |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-23 00:40:04 | ID requested | |
| ⏵ 1 | 2023-04-23 00:40:19 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cell, Proteome profiling, ZBED3 overexpression, Co-Immunoprecipitation |
Contact List
| Xiaohe Luo | |
|---|---|
| contact affiliation | Department of Laboratory Medicine, Chongqing University Three Gorges Hospital |
| contact email | xiaoheluo@163.com |
| lab head | |
| Xiaohe Luo | |
| contact affiliation | Chongqing University |
| contact email | xiaoheluo@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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