PXD041736

PXD041736 is an original dataset announced via ProteomeXchange.

Title	Label-free Quantification of Host-Cell Protein Impurity in a Recombinant Hemoglobin Reference Material
Description	Quantitative analysis depends on pure-substance primary calibrators with known mass fractions of impurity. The datasets included were generated using mass spectrometry for the evaluation of label-free quantification (LFQ) as a method for determining the mass fraction of host-cell proteins (HCPs) in bioengineered proteins. For this purpose, hemoglobin-A2 (HbA2) was used, as obtained by overexpression in E.coli. Two different materials had been produced: natural, and U-15N-labeled HbA2. To quantify impurity, precursor ion (MS1-) intensities were integrated over all E.coli-proteins identified and divided by the intensities obtained for HbA2. This ratio was calibrated against the corresponding results for an E.coli-cell lysate, that had been spiked at known mass-ratios to pure HbA2. To demonstrate the universal applicability of LFQ, further proteomes (yeast and human K562) were then alternatively used for calibration and were found to produce comparable results. Valid results were also obtained when the complexity of the calibrator was reduced to a mix of nine proteins.
HostingRepository	PRIDE
AnnounceDate	2023-12-12
AnnouncementXML	Submission_2023-12-12_08:18:37.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Arsene
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-22 13:06:16	ID requested
1	2023-05-09 14:19:55	announced
⏵ 2	2023-12-12 08:18:38	announced	2023-12-12: Updated project metadata.

10.1007/s00216-023-05024-8;

Henrion A, Arsene CG, Liebl M, O'Connor G, Label-free quantification of host cell protein impurity in recombinant hemoglobin materials. Anal Bioanal Chem, 416(2):387-396(2024) [pubmed]

submitter keyword: Protein Reference Materials, DDA-PASEF,Label-free Quantification, Host-Cell Proteins

Cristian Arsene
contact affiliation	Physikalisch-Technische Bundesanstalt (PTB), Braunschweig und Berlin, D-38116 Braunschweig, Germany
contact email	christian.arsene@ptb.de
lab head
Cristian Arsene
contact affiliation	Physikalisch-Technische Bundesanstalt (PTB)
contact email	christian.arsene@ptb.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD041736

PRIDE project URI

• PRIDE
  1. PXD041736
     1. Label: PRIDE project
     2. Name: Label-free Quantification of Host-Cell Protein Impurity in a Recombinant Hemoglobin Reference Material