PXD041723

PXD041723 is an original dataset announced via ProteomeXchange.

Title	DRMY1 promotes robust morphogenesis by sustaining translation of a hormone signaling protein
Description	Robustness is the invariant development of phenotype despite environmental changes and genetic perturbations. In the Arabidopsis flower bud, four sepals initiate at robust positions and times and grow to equal size to enclose and protect the inner floral organs. We previously characterized the mutant development related myb-like1 (drmy1), where 3-5 sepals initiate at irregular positions and variable times and grow to different sizes, compromising their protective function. The molecular mechanism underlying this loss of robustness was unclear. Here, we show that drmy1 has reduced TARGET OF RAPAMYCIN (TOR) activity, ribosomal content, and translation. Translation reduction decreases the protein level of ARABIDOPSIS RESPONSE REGULATOR7 (ARR7), a rapidly synthesized and degraded cytokinin signaling inhibitor. The resultant upregulation of cytokinin signaling disrupts the robust positioning of auxin signaling, causing variable sepal initiation. Our work shows that the homeostasis of translation, a ubiquitous cellular process, is crucial for the robust spatiotemporal patterning of organogenesis.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:32:02.129.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041723
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shuyao Kong
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-21 12:29:20	ID requested
⏵ 1	2025-12-07 16:32:03	announced

10.1016/j.devcel.2024.08.010;

Kong S, Zhu M, Scarpin MR, Pan D, Jia L, Martinez RE, Alamos S, Vadde BVL, Garcia HG, Qian SB, Brunkard JO, Roeder AHK, DRMY1 promotes robust morphogenesis in Arabidopsis by sustaining the translation of cytokinin-signaling inhibitor proteins. Dev Cell, 59(23):3141-3160.e7(2024) [pubmed]

10.6019/PXD041723;

submitter keyword: Arabidopsis, cytokinin, auxin, sepal, TOR,Robustness, morphogenesis, ARR, translation, ribosomopathy

Adrienne Roeder
contact affiliation	Weill Institute for Cell and Molecular Biology and Section of Plant Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA
contact email	ahr75@cornell.edu
lab head
Shuyao Kong
contact affiliation	Cornell University
contact email	sk3245@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041723
     1. Label: PRIDE project
     2. Name: DRMY1 promotes robust morphogenesis by sustaining translation of a hormone signaling protein