PXD041722

PXD041722 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human AC16 Cardiac Cells under Doxorubicin Treatment
Description	AC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells with primary human ventricular fibroblasts (Promocell) with or without TGFb stimulation for 24 hours. The induction of senescence with doxorubicin and lack of spontaneous senescence was verified using p21 immunoblots. For mass spectrometry, 25 µg of proteins were reduced, alkylated, and digested with trypsin using a filter-assisted protocol on a Pierce 10 K MWCO column. The peptides were then labeled using Tandem Mass Tag (TMT) 10-plex reagents (Thermo), combined, and separated by high-pH reversed phase separation (Thermo), then analyzed on an Orbitrap HF mass spectrometer.
HostingRepository	jPOST
AnnounceDate	2023-04-22
AnnouncementXML	Submission_2024-09-26_14:27:36.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Lau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT; L-methionine sulfoxide; TMT; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-21 12:10:04	ID requested
1	2023-04-21 12:10:52	announced
⏵ 2	2024-09-26 14:27:36	announced	2024-09-27: Updated PubMed.

Publication List

Keyword List

submitter keyword: AC16, HVCF, Doxorubicin, TMT

submitter keyword: AC16, HVCF, Doxorubicin, TMT

Contact List

Edward Lau
lab head
Edward Lau
contact affiliation	University of Colorado
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002133/