PXD041722
PXD041722 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human AC16 Cardiac Cells under Doxorubicin Treatment |
Description | AC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells with primary human ventricular fibroblasts (Promocell) with or without TGFb stimulation for 24 hours. The induction of senescence with doxorubicin and lack of spontaneous senescence was verified using p21 immunoblots. For mass spectrometry, 25 µg of proteins were reduced, alkylated, and digested with trypsin using a filter-assisted protocol on a Pierce 10 K MWCO column. The peptides were then labeled using Tandem Mass Tag (TMT) 10-plex reagents (Thermo), combined, and separated by high-pH reversed phase separation (Thermo), then analyzed on an Orbitrap HF mass spectrometer. |
HostingRepository | jPOST |
AnnounceDate | 2023-04-22 |
AnnouncementXML | Submission_2024-09-26_14:27:36.092.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Edward Lau |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT; L-methionine sulfoxide; TMT; S-carboxamidomethyl-L-cysteine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-04-21 12:10:04 | ID requested | |
1 | 2023-04-21 12:10:52 | announced | |
⏵ 2 | 2024-09-26 14:27:36 | announced | 2024-09-27: Updated PubMed. |
Publication List
Kastury N, Hidalgo V, Pandi B, Li L, Lam MPY, Lau E, Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss. MicroPubl Biol, 2023():(2023) [pubmed] |
Keyword List
submitter keyword: AC16, HVCF, Doxorubicin, TMT |
Contact List
Edward Lau | |
---|---|
lab head | |
Edward Lau | |
contact affiliation | University of Colorado |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002133/ |