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PXD041722

PXD041722 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman AC16 Cardiac Cells under Doxorubicin Treatment
DescriptionAC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells with primary human ventricular fibroblasts (Promocell) with or without TGFb stimulation for 24 hours. The induction of senescence with doxorubicin and lack of spontaneous senescence was verified using p21 immunoblots. For mass spectrometry, 25 µg of proteins were reduced, alkylated, and digested with trypsin using a filter-assisted protocol on a Pierce 10 K MWCO column. The peptides were then labeled using Tandem Mass Tag (TMT) 10-plex reagents (Thermo), combined, and separated by high-pH reversed phase separation (Thermo), then analyzed on an Orbitrap HF mass spectrometer.
HostingRepositoryjPOST
AnnounceDate2023-04-22
AnnouncementXMLSubmission_2024-09-26_14:27:36.092.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEdward Lau
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT; L-methionine sulfoxide; TMT; S-carboxamidomethyl-L-cysteine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-21 12:10:04ID requested
12023-04-21 12:10:52announced
22024-09-26 14:27:36announced2024-09-27: Updated PubMed.
Publication List
Kastury N, Hidalgo V, Pandi B, Li L, Lam MPY, Lau E, Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss. MicroPubl Biol, 2023():(2023) [pubmed]
Keyword List
submitter keyword: AC16, HVCF, Doxorubicin, TMT
Contact List
Edward Lau
lab head
Edward Lau
contact affiliationUniversity of Colorado
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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