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PXD041710

PXD041710 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNucleoporin Nsp1 surveils the phase state of FG-Nups
DescriptionTransport through the NPC relies on intrinsically disordered FG-Nups forming a selective barrier. Away from the NPC, FG-Nups readily form condensates and aggregates, and we address how this behavior is surveilled in cells. FG-Nups, including Nsp1, together with nuclear transport receptor Kap95, form a native cytosolic condensate in yeast. In aged cells this condensate disappears as cytosolic Nsp1 levels decline. Biochemical assays and modeling show that Nsp1 is a modulator of FG-Nup liquid-liquid phase separation, promoting a liquid-like state. Nsp1’s presence in the cytosol and condensates is critical, as a reduction of cytosolic levels in young cells induces NPC assembly and transport defects and a general decline in protein quality control, all quantitatively mimicking aging phenotypes. Excitingly, these phenotypes can be rescued by cytosolic Nsp1. We conclude that Nsp1 is a phase state regulator that surveils FG-Nups and impacts general protein homeostasis.
HostingRepositoryPRIDE
AnnounceDate2025-01-27
AnnouncementXMLSubmission_2025-01-27_01:30:39.115.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJustina Clarinda Wolters
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-21 01:18:54ID requested
12025-01-27 01:30:39announced
Publication List
10.1016/J.CELREP.2024.114793;
Keyword List
submitter keyword: Liquid-liquid phase-separation,Nuclear Pore Complex, Chaperones, Condensate, Aging, Protein homeostasis, Intrinsically Disordered Protein, Nsp1, FG-nucleoporin, Kap95
Contact List
Justina Clarinda Wolters
contact affiliationDepartment of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
contact emailj.c.wolters@umcg.nl
lab head
Justina Clarinda Wolters
contact affiliationUMCG
contact emailj.c.wolters@umcg.nl
dataset submitter
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Dataset FTP location
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