PXD041710

PXD041710 is an original dataset announced via ProteomeXchange.

Title	Nucleoporin Nsp1 surveils the phase state of FG-Nups
Description	Transport through the NPC relies on intrinsically disordered FG-Nups forming a selective barrier. Away from the NPC, FG-Nups readily form condensates and aggregates, and we address how this behavior is surveilled in cells. FG-Nups, including Nsp1, together with nuclear transport receptor Kap95, form a native cytosolic condensate in yeast. In aged cells this condensate disappears as cytosolic Nsp1 levels decline. Biochemical assays and modeling show that Nsp1 is a modulator of FG-Nup liquid-liquid phase separation, promoting a liquid-like state. Nsp1’s presence in the cytosol and condensates is critical, as a reduction of cytosolic levels in young cells induces NPC assembly and transport defects and a general decline in protein quality control, all quantitatively mimicking aging phenotypes. Excitingly, these phenotypes can be rescued by cytosolic Nsp1. We conclude that Nsp1 is a phase state regulator that surveils FG-Nups and impacts general protein homeostasis.
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_01:30:39.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justina Clarinda Wolters
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-21 01:18:54	ID requested
⏵ 1	2025-01-27 01:30:39	announced

10.1016/J.CELREP.2024.114793;

submitter keyword: Liquid-liquid phase-separation,Nuclear Pore Complex, Chaperones, Condensate, Aging, Protein homeostasis, Intrinsically Disordered Protein, Nsp1, FG-nucleoporin, Kap95

Justina Clarinda Wolters
contact affiliation	Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
contact email	j.c.wolters@umcg.nl
lab head
Justina Clarinda Wolters
contact affiliation	UMCG
contact email	j.c.wolters@umcg.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041710
     1. Label: PRIDE project
     2. Name: Nucleoporin Nsp1 surveils the phase state of FG-Nups