PXD041710 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nucleoporin Nsp1 surveils the phase state of FG-Nups |
Description | Transport through the NPC relies on intrinsically disordered FG-Nups forming a selective barrier. Away from the NPC, FG-Nups readily form condensates and aggregates, and we address how this behavior is surveilled in cells. FG-Nups, including Nsp1, together with nuclear transport receptor Kap95, form a native cytosolic condensate in yeast. In aged cells this condensate disappears as cytosolic Nsp1 levels decline. Biochemical assays and modeling show that Nsp1 is a modulator of FG-Nup liquid-liquid phase separation, promoting a liquid-like state. Nsp1’s presence in the cytosol and condensates is critical, as a reduction of cytosolic levels in young cells induces NPC assembly and transport defects and a general decline in protein quality control, all quantitatively mimicking aging phenotypes. Excitingly, these phenotypes can be rescued by cytosolic Nsp1. We conclude that Nsp1 is a phase state regulator that surveils FG-Nups and impacts general protein homeostasis. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-27 |
AnnouncementXML | Submission_2025-01-27_01:30:39.115.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Justina Clarinda Wolters |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-21 01:18:54 | ID requested | |
⏵ 1 | 2025-01-27 01:30:39 | announced | |
Publication List
Keyword List
submitter keyword: Liquid-liquid phase-separation,Nuclear Pore Complex, Chaperones, Condensate, Aging, Protein homeostasis, Intrinsically Disordered Protein, Nsp1, FG-nucleoporin, Kap95 |
Contact List
Justina Clarinda Wolters |
contact affiliation | Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands. |
contact email | j.c.wolters@umcg.nl |
lab head | |
Justina Clarinda Wolters |
contact affiliation | UMCG |
contact email | j.c.wolters@umcg.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041710
- Label: PRIDE project
- Name: Nucleoporin Nsp1 surveils the phase state of FG-Nups