PXD041709

PXD041709 is an original dataset announced via ProteomeXchange.

Title	Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia gene intraflagellar transport homolog 88 in Alzheimer's disease
Description	A proteomic analysis of synaptic compartments obtained from septal-projecting hippocampal neurons revealed the formation of dystrophic neurites and synaptic loss at axon terminals as a result of the accumulation of numerous vesicles. Subsequent analysis also showed that toxic amyloid-β oligomers in the vicinity of dystrophic axons affected primary cilia, vesicle endocytosis, and neuronal exocytosis. The proteomic analysis of extracellular vesicles in presynaptic terminals confirmed that abnormal vesicle-mediated transport and exocytosis led to the accumulation of amyloid-β with extracellular vesicles at axon terminals. Further investigation showed that knockdown of the intraflagellar transport homolog 88 gene, which is essential for primary ciliogenesis in hippocampal neurons, led to an increase in amyloid-β uptake, leading to their accumulation with other synaptic vesicles at axon terminals.
HostingRepository	PRIDE
AnnounceDate	2023-05-17
AnnouncementXML	Submission_2023-05-16_17:20:29.529.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JaemyungJang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-21 01:09:45	ID requested
⏵ 1	2023-05-16 17:20:29	announced

Dataset with its publication pending

submitter keyword: primary cilia, extracellular vesicles, intraflagellar transport homolog 88, dystrophic axons

YoungshikChoe
contact affiliation	Korea Brain Research Institute, Daegu, 41062, South Korea
contact email	dallarae@gmail.com
lab head
JaemyungJang
contact affiliation	Korea Brain Research Institute
contact email	piloter2@kbri.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD041709

PRIDE project URI

• PRIDE
  1. PXD041709
     1. Label: PRIDE project
     2. Name: Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia gene intraflagellar transport homolog 88 in Alzheimer's disease