PXD041702

PXD041702 is an original dataset announced via ProteomeXchange.

Title	Atg9 resides on a unique population of small vesicles in presynaptic nerve terminals
Description	In neurons, autophagosomes are mainly initiated in distal axons, followed by maturation during retrograde transport. Autophagosomal growth depends on the supply of membrane lipids which requires small vesicles containing Atg9, a lipid scramblase essential for autophagocytosis. Here, we show that Atg9 vesicles are enriched in synapses and resemble synaptic vesicles in size and density. The proteome of Atg9 vesicles immunoisolated from nerve terminals showed conspicuously low levels of trafficking proteins except of the AP2-complex and some enzymes involved in endosomal phosphatidylinositol metabolism. Super resolution microscopy of nerve terminals and isolated vesicles revealed that Atg9-vesicles represent a distinct vesicle population with limited overlap not only with synaptic vesicles but also other membranes of the secretory pathway, uncovering a surprising heterogeneity in their membrane composition. Our results are compatible with the view that Atg9-vesicles function as lipid shuttles that scavenge membrane lipids from various intracellular membranes to support autophagosome biogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:23:48.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandar Chernev
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 19:09:14	ID requested
1	2024-01-17 23:11:23	announced
⏵ 2	2024-10-22 06:23:48	announced	2024-10-22: Updated project metadata.

10.1080/15548627.2023.2274204;

submitter keyword: neurons, immunoisolation,Atg9

Henning Urlaub
contact affiliation	Max-Planck-Institute for Multidisciplinary Sciences Bioanalytical Mass Spectrometry Group Am Fassberg 11 D-37077 Goettingen, Germany Bioanalytics Institute for Clinical Chemistry Robert Koch Strasse 40 D-37075 Goettingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Aleksandar Chernev
contact affiliation	MPIbpc
contact email	aleksandar.chernev@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD041702

PRIDE project URI

• PRIDE
  1. PXD041702
     1. Label: PRIDE project
     2. Name: Atg9 resides on a unique population of small vesicles in presynaptic nerve terminals