PXD041699

PXD041699 is an original dataset announced via ProteomeXchange.

Title	ATM-knockout on total and phosphorylation proteomes of cortical brain organoids
Description	ATM (ataxia telangiectasia mutated) kinase is crucial to a wide range of human developmental disorders and adult/pediatric malignancies. Its mutations are causally tied to ataxia telangiectasia, a multi-systemic congenital disorder mainly affecting brain and blood systems. We generated 4 separate ATM-knockout human pluripotent stem cell lines and differentiated them to form 3-dimensional brain cortical brain organoids. Brain cortical organoids are an excellent model of human developing cortex. Using these analyses, we identified ATM-dependent phosphorylation predominantly influences factors in neurogenesis, neuronal differentiation, cell morphogenesis, and microtubule cytoskeleton as well as kinases involved in ATM, BNDF, and WNT signaling, G2/M checkpoint, and p53 regulation. These findings have broad implications about diseases associated with ATM, including ataxia telangiectasia.
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_09:58:31.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vishwajeeth Pagala
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 16:13:56	ID requested
⏵ 1	2024-07-25 09:58:32	announced

10.1101/2023.05.04.539457;

submitter keyword: phosphorylation proteome

proteome

ATM kinase

cortical brain organoids

Jamy Peng
contact affiliation	Associate Member Developmental Neurobiology St. Jude Children's Research Hospital
contact email	jamy.peng@stjude.org
lab head
Vishwajeeth Pagala
contact affiliation	St. Jude Children's Research Hospital
contact email	vishwajeeth.pagala@stjude.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041699
     1. Label: PRIDE project
     2. Name: ATM-knockout on total and phosphorylation proteomes of cortical brain organoids