PXD041698
PXD041698 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | DSSO Crosslinking of IEX column fractions from Tetrahymena cilia extract fractionation. |
| Description | Procedure details are reported in McCafferty et al. eLife2022;11e81977. Briefly cilia extract from Tetrahymena thermophila SB715 was fractionated on a mixed bed IEX HPLC column and all resulting fractions were crosslinked by addition of DSSO to 0.5mM. Following reduction, alkylation, trypsin digestion, and desalting, mass spectra were collected on an Orbitrap Fusion Lumos using a DDA MS2-MS3 method and spectra were processed in ProteomeDiscoverer 2.3 using the Xlinkx node to identify crosslinks. The look-up database for crosslink identification was generated from the same .RAW files using a standard Basic workflow to identify proteins present in the fractions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-06 |
| AnnouncementXML | Submission_2024-03-06_11:09:23.484.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ophelia Papoulas |
| SpeciesList | scientific name: Tetrahymena thermophila; NCBI TaxID: 5911; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-20 15:31:43 | ID requested | |
| ⏵ 1 | 2024-03-06 11:09:23 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Tetrahymena thermophila, DSSO, Crosslink, IEX, cilia |
Contact List
| Edward Marcotte | |
|---|---|
| contact affiliation | University of Texas-Austin |
| contact email | marcotte@icmb.utexas.edu |
| lab head | |
| Ophelia Papoulas | |
| contact affiliation | University of Texas at Austin |
| contact email | papoulas@austin.utexas.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000091761/ |
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