PXD041695

PXD041695 is an original dataset announced via ProteomeXchange.

Title	Convergent origin and accelerated evolution of vesicle-associated RhoGAP proteins in two unrelated parasitoid wasps
Description	Gene duplication, accompanied by modification of the expression and/or function of one of the duplicates under the action of positive selection, followed by further duplication to produce multigene families of toxins is a well-documented process in venomous animals. This evolutionary model has been less described in parasitoid wasps, which use maternal fluids, including venom, to protect their eggs from encapsulation by the host immune system. The leptopilina venom proteomic data were used to evidence that specific RhoGAPs formed a family of protein that are associated with vesicles that act as transport systems to deliver them in the immune cells of their drosophila larval host. We showed that the gene encoding the cellular RacGAP1 is at the origin of the virulent RhoGAP family formed by successive duplications that evolved under positive selection. Almost all of these RhoGAPs lost their GAP activity and GTPase binding ability due to mutations in key amino acids suggesting new function(s) and mechanism of action in host cells that remain to be elucidated.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:14:06.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maya BELGHAZI
SpeciesList	scientific name: Leptopilina boulardi; NCBI TaxID: 63433; scientific name: Leptopilina heterotoma; NCBI TaxID: 63436;
ModificationList	iodoacetamide derivatized residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 14:30:14	ID requested
⏵ 1	2024-10-08 11:14:07	announced

10.1016/j.ibmb.2013.03.010;

Colinet D, Deleury E, Anselme C, Cazes D, Poulain J, Azema-Dossat C, Belghazi M, Gatti JL, Poiri, é M, Extensive inter- and intraspecific venom variation in closely related parasites targeting the same host: the case of Leptopilina parasitoids of Drosophila. Insect Biochem Mol Biol, 43(7):601-11(2013) [pubmed]

submitter keyword: gene duplication, venom, evolution, positive selection,parasitoids, RhoGAP, ovarian calyx

Jean Luc gatti
contact affiliation	INRAE Evolution and Specificity of Multitrophic Interactions (ESIM), Sophia Agrobiotech Institute (ISA) 400 route des Chappes, Sophia Antipolis 06903, France
contact email	jean-luc.gatti@inrae.fr
lab head
Maya BELGHAZI
contact affiliation	CNRS
contact email	maya.belghazi@univ-amu.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041695

PRIDE project URI

• PRIDE
  1. PXD041695
     1. Label: PRIDE project
     2. Name: Convergent origin and accelerated evolution of vesicle-associated RhoGAP proteins in two unrelated parasitoid wasps