PXD041693

PXD041693 is an original dataset announced via ProteomeXchange.

Title	Metabolic interplay between Proteus mirabilis and Enterococcus faecalis facilitates polymicrobial biofilm formation and invasive disease
Description	Catheter-associated urinary tract infections (CAUTI) account for 40% of all nosocomial infections and can lead to significant life-threatening complications such as bacteremia. Microbial biofilms play an important role in the development and pathogenesis of CAUTI, and these biofilms are often polymicrobial. Proteus mirabilis and Enterococcus faecalis are two of the most common causes of CAUTI, and they often persistently co-colonize the catheterized urinary tract. We previously demonstrated that co-culture of E. faecalis with P. mirabilis increased biofilm biomass, antimicrobial resistance, and disease severity. In this study, we uncover the metabolic interplay that drives biofilm enhancement and examine the contribution of this polymicrobial interaction to CAUTI severity. Through compositional and proteomic biofilm analyses, we determined that the increase in biofilm biomass stems from an increase in the protein fraction of the polymicrobial biofilm matrix. We further observed an enrichment in proteins associated with ornithine and arginine metabolism in polymicrobial biofilms compared to single-species biofilms. By testing mutants of E. faecalis and P. mirabilis, we found that L-ornithine secreted by the E. faecalis ArcD antiporter promotes L-arginine biosynthesis in P. mirabilis via ArgF, which ultimately fuels production of proteins that facilitate contact-dependent interactions to enhance biofilm biomass. We further demonstrate that ArcD and ArgF are not important for urinary tract colonization by either species when alone, but ornithine/arginine interplay is critical for the increased disease severity that occurs during coinfection. This study provides deeper insight into the polymicrobial interactions occurring during CAUTI and highlights how these interactions can have significant impacts on pathogenesis and bacterial persistence.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:31:21.145.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shichen Shen
SpeciesList	scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Proteus mirabilis HI4320; NCBI TaxID: 529507;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 11:41:57	ID requested
⏵ 1	2025-05-06 09:31:22	announced

10.1128/mbio.02164-24;

Hunt BC, Brix V, Vath J, Guterman LB, Taddei SM, Deka N, Learman BS, Brauer AL, Shen S, Qu J, Armbruster CE, facilitates polymicrobial biofilm formation and invasive disease. mBio, 15(12):e0216424(2024) [pubmed]

submitter keyword: IonStar, Enterococcus faecalis,Proteus mirabilis, quantitative proteomics, biofilm

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, School of Phamarcy and Pharmaceutical Sciences, SUNY at Buffalo
contact email	junqu@buffalo.edu
lab head
Shichen Shen
contact affiliation	University at Buffalo
contact email	shichens@buffalo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041693
     1. Label: PRIDE project
     2. Name: Metabolic interplay between Proteus mirabilis and Enterococcus faecalis facilitates polymicrobial biofilm formation and invasive disease