PXD041690

PXD041690 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	O-Glycosylation of Mycobacteriophage Proteins
Description	Provided herein is the first evidence that mycobacteriophage, indeed phage of any kind, have surface proteins (capsid and/or tail tube proteins) that are O-glycosylated. The glycosyltranferases that glycosylate these proteins are encoded in the phage genomes. In addition, for the three mycobacteriophages invested in this study, the glycans appear to be attached exclusively at a C-terminal serine residue. Each phage expresses a unique and novel O-glycan. The LC-MS files deposited here are the preliminary evidence that these mycobacteriophage proteins are glycosylated. A follow-up investigation will examine the composition and structure of the O-glycans in more detail.
HostingRepository	PRIDE
AnnounceDate	2024-08-10
AnnouncementXML	Submission_2024-08-10_12:05:42.927.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041690
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Kelly
SpeciesList	scientific name: Mycobacterium phage Myrna; NCBI TaxID: 546805; scientific name: Mycobacterium phage Corndog; NCBI TaxID: 2907833; scientific name: Mycobacterium phage Che8; NCBI TaxID: 2907829;
ModificationList	complex glycosylation
Instrument	Orbitrap Eclipse; Q-Tof Premier; Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 09:39:13	ID requested
⏵ 1	2024-08-10 12:05:43	announced

Publication List

10.1016/j.chom.2023.05.028;
Freeman KG, Robotham AC, Parks OB, Abad L, Jacobs-Sera D, Lauer MJ, Podgorski JM, Zhang Y, Williams JV, White SJ, Kelly JF, Hatfull GF, Pope WH, Virion glycosylation influences mycobacteriophage immune recognition. Cell Host Microbe, 31(7):1216-1231.e6(2023) [pubmed]
10.6019/PXD041690;

10.1016/j.chom.2023.05.028;

Freeman KG, Robotham AC, Parks OB, Abad L, Jacobs-Sera D, Lauer MJ, Podgorski JM, Zhang Y, Williams JV, White SJ, Kelly JF, Hatfull GF, Pope WH, Virion glycosylation influences mycobacteriophage immune recognition. Cell Host Microbe, 31(7):1216-1231.e6(2023) [pubmed]

10.6019/PXD041690;

Keyword List

submitter keyword: O-glycosylation, Tail Tube Protein,Mycobacteriophage, Capsid

submitter keyword: O-glycosylation, Tail Tube Protein,Mycobacteriophage, Capsid

Contact List

John Kelly
contact affiliation	Human Health Therapeutics, National Research Council of Canada
contact email	John.Kelly@nrc-cnrc.gc.ca
lab head
John Kelly
contact affiliation	Human Health Therapeutics, National Research Council of Canada
contact email	John.Kelly@nrc-cnrc.gc.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD041690
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD041690

PRIDE project URI

Repository Record List

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