PXD041683

PXD041683 is an original dataset announced via ProteomeXchange.

Title	Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1
Description	The 22Rv1 and PC-3 cells were transduced with shMIMIC human lentiviral vectors (Horizon Discovery, Cambridge, UK) to stably overexpress miRNA-23c or -4328. The SMARTvector Non-Targeting Control (NTC) was expressed to serve as a negative control. The vectors contained a turbo green fluorescent protein (turboGFP) and a puromycin resistance gene cassette. After transduction, cells were cultured in a medium supplemented with 5 µg/mL puromycin (Takara Bio, Tokyo, Japan) for antibiotic selection. Overexpression was confirmed by monitoring the turboGFP by fluorescence microscopy and by RT-qPCR analysis of miRNA-23c and -4328 levels. Relative protein quantification was performed to compare protein expression in 22Rv1 and PC-3 single cell clones overexpressing miRNA-23c and -4328, compared to corresponding NTC cells. Single cell clones overexpressing either miRNA-23c (n = 3), -4328 (n = 3) or NTC (n = 3) were analyzed in triplicate.
HostingRepository	PRIDE
AnnounceDate	2023-04-24
AnnouncementXML	Submission_2023-04-24_02:13:29.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PernillaWikström
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 07:17:42	ID requested
⏵ 1	2023-04-24 02:13:30	announced

Dataset with its publication pending

submitter keyword: Human, Overexpression, Prostate cancer, MircoRNA, Cell lines, nano LC-MS/MS

PernillaWikström
contact affiliation	Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
contact email	pernilla.wikstrom@umu.se
lab head
PernillaWikström
contact affiliation	Umeå University
contact email	pernilla.wikstrom@umu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD041683

PRIDE project URI

• PRIDE
  1. PXD041683
     1. Label: PRIDE project
     2. Name: Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1