PXD041683 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1 |
Description | The 22Rv1 and PC-3 cells were transduced with shMIMIC human lentiviral vectors (Horizon Discovery, Cambridge, UK) to stably overexpress miRNA-23c or -4328. The SMARTvector Non-Targeting Control (NTC) was expressed to serve as a negative control. The vectors contained a turbo green fluorescent protein (turboGFP) and a puromycin resistance gene cassette. After transduction, cells were cultured in a medium supplemented with 5 µg/mL puromycin (Takara Bio, Tokyo, Japan) for antibiotic selection. Overexpression was confirmed by monitoring the turboGFP by fluorescence microscopy and by RT-qPCR analysis of miRNA-23c and -4328 levels. Relative protein quantification was performed to compare protein expression in 22Rv1 and PC-3 single cell clones overexpressing miRNA-23c and -4328, compared to corresponding NTC cells. Single cell clones overexpressing either miRNA-23c (n = 3), -4328 (n = 3) or NTC (n = 3) were analyzed in triplicate. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-24 |
AnnouncementXML | Submission_2023-04-24_02:13:29.858.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | PernillaWikström |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-20 07:17:42 | ID requested | |
⏵ 1 | 2023-04-24 02:13:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, Overexpression, Prostate cancer, MircoRNA, Cell lines, nano LC-MS/MS |
Contact List
PernillaWikström |
contact affiliation | Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden |
contact email | pernilla.wikstrom@umu.se |
lab head | |
PernillaWikström |
contact affiliation | Umeå University |
contact email | pernilla.wikstrom@umu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041683
- Label: PRIDE project
- Name: Protein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1