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PXD041683

PXD041683 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein profiles following miRNA-23c and -4328 overexpression in prostate cancer cell lines, PC-3 and 22Rv1
DescriptionThe 22Rv1 and PC-3 cells were transduced with shMIMIC human lentiviral vectors (Horizon Discovery, Cambridge, UK) to stably overexpress miRNA-23c or -4328. The SMARTvector Non-Targeting Control (NTC) was expressed to serve as a negative control. The vectors contained a turbo green fluorescent protein (turboGFP) and a puromycin resistance gene cassette. After transduction, cells were cultured in a medium supplemented with 5 µg/mL puromycin (Takara Bio, Tokyo, Japan) for antibiotic selection. Overexpression was confirmed by monitoring the turboGFP by fluorescence microscopy and by RT-qPCR analysis of miRNA-23c and -4328 levels. Relative protein quantification was performed to compare protein expression in 22Rv1 and PC-3 single cell clones overexpressing miRNA-23c and -4328, compared to corresponding NTC cells. Single cell clones overexpressing either miRNA-23c (n = 3), -4328 (n = 3) or NTC (n = 3) were analyzed in triplicate.
HostingRepositoryPRIDE
AnnounceDate2023-04-24
AnnouncementXMLSubmission_2023-04-24_02:13:29.858.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPernillaWikström
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-04-20 07:17:42ID requested
12023-04-24 02:13:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, Overexpression, Prostate cancer, MircoRNA, Cell lines, nano LC-MS/MS
Contact List
PernillaWikström
contact affiliationDepartment of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden
contact emailpernilla.wikstrom@umu.se
lab head
PernillaWikström
contact affiliationUmeå University
contact emailpernilla.wikstrom@umu.se
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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