PXD041681

PXD041681 is an original dataset announced via ProteomeXchange.

Title	Changes in the proteome of platelets from patients with critical progression of COVID-19
Description	Platelets, the smallest cells in human blood known for their role in primary haemostasis, are also able to interact with pathogens and play a crucial role in the immune response. In severe coronavirus disease 2019 (COVID-19) cases, platelets become over-activated, resulting in the release of granules, exacerbating inflammation and contributing to the cytokine storm. Here we utilize label-free shotgun proteomics approach to further elucidate the role of platelets in COVID-19 progression and to identify predictive biomarkers for disease outcome. Platelet proteome of the severely ill COVID-19 patients varied significantly from to healthy controls. Significant changes in the levels of proteins associated with protein folding were detected. In addition, a number of proteins with isomerase activity were found in the patient samples, which appear to exert an influence on platelet activity via non-genomic properties of glucocorticoid receptor (GR) and nuclear factor κ-light-chain-enhancer of activated B cells (NFkB). We also performed a comparison of proteins found exclusively in controls, survivors and non-survivors. CRP, CA-1, SAA2 and SEPPINA3 were found to be biomarker candidates in platelets, showing a significant increase in ICU patients.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:34.760.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041681
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Svitlana Rozanova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-20 06:15:11	ID requested
1	2023-10-24 12:46:55	announced
⏵ 2	2023-11-14 09:10:35	announced	2023-11-14: Updated project metadata.

10.6019/PXD041681;

Wolny M, Rozanova S, Knabbe C, Pfeiffer K, Barkovits K, Marcus K, Birschmann I, Changes in the Proteome of Platelets from Patients with Critical Progression of COVID-19. Cells, 12(17):(2023) [pubmed]

submitter keyword: proteomics, glucocorticoid re-ceptor, ECMO, inflammation, NFkB, acute-phase proteins, carbonic anhydrase 1, COVID-19,platelets

Prof. Katrin Marcus-Alic
contact affiliation	Medizinisches Proteom-Center, Medical Faculty, Ruhr University Bochum, 44801 Bochum, Germany
contact email	katrin.marcus@ruhr-uni-bochum.de
lab head
Svitlana Rozanova
contact affiliation	Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center
contact email	Svitlana.Rozanova@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD041681

PRIDE project URI

• PRIDE
  1. PXD041681
     1. Label: PRIDE project
     2. Name: Changes in the proteome of platelets from patients with critical progression of COVID-19