PXD041655

PXD041655 is an original dataset announced via ProteomeXchange.

Title	A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors
Description	Information processing and storage in the brain rely on AMPA-receptors (AMPARs) and their context-dependent dynamics in synapses and extra-synaptic sites. We found that distribution and dynamics of AMPARs in the plasma membrane are controlled by Noelins, a three-member family of conserved secreted proteins expressed throughout the brain in a cell type-specific manner. Noelin tetramers tightly assemble with the extracellular domains of AMPARs and interconnect them in a network-like configuration with a variety of secreted and membrane-anchored proteins including Neurexin1, Neuritin1, and Seizure 6-like. Knockout of Noelins1-3 profoundly reduced AMPARs in synapses onto excitatory and inhibitory (inter)neurons, decreased their density and clustering in dendrites and abolished activity-dependent synaptic plasticity. Our results uncover an endogenous mechanism for extracellular anchoring of AMPARs and establish Noelin-organized networks as versatile determinants of constitutive and context-dependent neurotransmission.
HostingRepository	PRIDE
AnnounceDate	2024-03-14
AnnouncementXML	Submission_2024-03-13_17:24:56.073.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041655
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Haupt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-19 10:14:49	ID requested
⏵ 1	2024-03-13 17:24:57	announced

10.6019/PXD041655;

Boudkkazi S, Schwenk J, Nakaya N, Brechet A, Kollewe A, Harada H, Bildl W, Kulik A, Dong L, Sultana A, Zolles G, Schulte U, Tomarev S, Fakler B, A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors. Neuron, 111(16):2544-2556.e9(2023) [pubmed]

10.1016/j.neuron.2023.07.013;

submitter keyword: AMPA receptors, Proteomics,Molecular Neuroscience

Prof. Dr. Bernd Fakler
contact affiliation	Institute of Physiology II, University of Freiburg, Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliation	University of Freiburg, Germany
contact email	alexander.haupt@physiologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD041655

PRIDE project URI

• PRIDE
  1. PXD041655
     1. Label: PRIDE project
     2. Name: A Noelin-organized extracellular network of proteins required for constitutive and context-dependent anchoring of AMPA-receptors