PXD041632

PXD041632 is an original dataset announced via ProteomeXchange.

Title	Mapping the Proteome Landscape of Indian Zebu (Sahiwal) Spermatozoa using High-Resolution Mass Spectrometry and In-silico Annotation
Description	Proteome of sperm cells provides earnest information related to protein structure, function, ex-pression, interactions, and localization in biological systems. Sahiwal is an indigenous Indian cat-tle breed that is acknowledged for its disease resistance, ease of calving, and drought resilience. The lack of any detailed data pertaining to comprehensive proteome profiling of Sahiwal sperms was the major objective behind this study. We conducted the first global in-silico quantitative high-resolution mass spectrometry-based Indian Zebu sperm protein profiling and identified a to-tal of 4651 sperm proteins. Moreover, we characterized the identified proteins at the sub-organellar level for a better understanding of their functional aspect. Gene Ontology analysis of sperm proteins segregated the proteins based on function, location, and mode of action. The study revealed that even though sperm contains extremely few organelles, evolution has struc-tured essentially all-important proteins in a single cell, despite their organellar specialization. It was also seen that sperm compensates for its lack of organelles by the presence of multitasking proteins. Almost all sperm proteins play an active role in spermatogenesis, motility, acrosome re-action, capacitation, and seminal plasma binding either directly or indirectly. Our results not only report the highest number of identified bovine sperm proteins, but also potentially serve as a foundation for empirical work on sperm functions, egg-sperm interaction, sperm-sex sorting bi-omarkers, sperm quality, and bull fertility.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:57:50.327.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Syed Azmal Ali
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-18 14:14:29	ID requested
⏵ 1	2025-12-14 16:57:51	announced

Bhushan V, Ali SA, Parashar A, Kumar S, Mohanty AK, annotation. Anim Biotechnol, 35(1):2428402(2024) [pubmed]

10.1080/10495398.2024.2428402;

submitter keyword: sperm

Ashok Mohanty
contact affiliation	National Dairy Research Institute
contact email	azmal786@gmail.com
lab head
Syed Azmal Ali
contact affiliation	German Cancer Research Center
contact email	azmal786@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041632
     1. Label: PRIDE project
     2. Name: Mapping the Proteome Landscape of Indian Zebu (Sahiwal) Spermatozoa using High-Resolution Mass Spectrometry and In-silico Annotation