PXD041631

PXD041631 is an original dataset announced via ProteomeXchange.

Title	Mucin-type O-glycans enrich in white matter tracts
Description	Protein O-glycosylation, the enzymatic attachment of carbohydrates to serine/threonine/tyrosine residues, is a large group of essential post-translational modifications involved in brain development and disease. However, studies of the most abundant O-glycan class in the mammalian brain, termed mucins or O-GalNAc glycans, are limited. Here, we determined the cellular specificity, spatial distribution, and protein carriers of O-GalNAc glycans in the mouse brain. Genetic inhibition of the O-GalNAc pathway in neurons or astrocytes had minimal effect on total O-GalNAc levels in the brain and failed to replicate the severe neurologic phenotype seen in rodent models and human congenital disorders of O-GalNAc synthesis. Further investigations revealed a surprising enrichment of O-GalNAc structures in white matter tracts and a spatial distribution distinct from other major carbohydrate structures in the brain including N-glycans. Using glycoproteomics, O-GalNAc glycans were identified on secretory proteins expressed in multiple cell types involved in brain development, synapse organization, and the extracellular matrix, including classical carriers of O-mannose glycans and proteoglycans. These findings highlight an overlooked aspect of O-GalNAc biology in white matter tracts of the brain and will inform future studies of development and disease.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_09:46:45.901.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suttipong Suttapitugsakul
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-18 12:29:36	ID requested
⏵ 1	2025-02-10 09:46:46	announced

Dataset with its publication pending

submitter keyword: Glycoproteomics

PNA

O-glycans

Robert G. Mealer
contact affiliation	Department of Psychiatry, Oregon Health & Science University, Portland, OR
contact email	mealer@ohsu.edu
lab head
Suttipong Suttapitugsakul
contact affiliation	Beth Israel Deaconess Medical Center and Harvard Medical School
contact email	ssuttapi@bidmc.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041631
     1. Label: PRIDE project
     2. Name: Mucin-type O-glycans enrich in white matter tracts