PXD041622

PXD041622 is an original dataset announced via ProteomeXchange.

Title	Identification of co-factors of eIF4E1b protein in mouse ovary.
Description	We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. eIF4E1b expresses specifically in mouse ovary and we have generated a knock-in mouse line in which HA tag is conjugated to the C terminus of eIF4E1b. We aim to identify eIF4E1b co-factors using mass spectrometry after anit-HA immunoprecipitation (IP) using mouse ovaries.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:47:49.105.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041622
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Anderson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-18 04:14:46	ID requested
1	2023-06-12 05:18:02	announced
2	2023-11-14 09:00:54	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:47:49	announced	2024-10-22: Updated project metadata.

10.1101/gad.350400.123;

10.6019/PXD041622;

Yang G, Xin Q, Feng I, Wu D, Dean J, Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation. Genes Dev, 37(9-10):418-431(2023) [pubmed]

submitter keyword: Mouse, Initiation facto, eIF-4E1b, affinity, ovary

Jurrien Dean
contact affiliation	Laboratory of Cellular and Developmental Biology, NIDDK, NIH, US Government
contact email	jurrien.dean@nih.gov
lab head
David Anderson
contact affiliation	NIH/NIDDK
contact email	davida@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD041622

PRIDE project URI

• PRIDE
  1. PXD041622
     1. Label: PRIDE project
     2. Name: Identification of co-factors of eIF4E1b protein in mouse ovary.