PXD041601
PXD041601 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identify the newly synthesized proteins by P. aeruginosa after engulfment by host immune cells by pulsed-SILAC proteomics |
| Description | To identify the newly synthesized proteins by P. aeruginosa after engulfment by host immune cells, we employed the pulsed-SILAC proteomics approach to label the bacterial proteome with L-lysine-13C6 15N2 (Lys8) and L-lysine (Lys0) before and after entering host cells, respectively. The PAO1â–³lysA mutant which failed to synthesize lysine was cultured in ABTG medium 37 supplemented with stable isotope-containing amino acid L-lysine-13C6 15N2 (Lys8). The Lys8 labeled bacteria were then harvested and used to infect the RAW264.7 cells which were cultured in DMEM containing regular L-lysine (Lys0). After 4 h infection, RAW264.7 cells were lysed, and the intracellular bacteria were harvested and analyzed by LC-MS/MS. For the control DMEM group, labeled bacteria were diluted in pure DMEM medium. By using this pulsed-labelling approach, the newly synthesized proteins can be selectively tagged with Lys0 during the infection. |
| HostingRepository | iProX |
| AnnounceDate | 2023-04-18 |
| AnnouncementXML | Submission_2023-04-17_20:30:04.845.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiangke Duan |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
| ModificationList | (13)C labeled residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-17 20:29:00 | ID requested | |
| ⏵ 1 | 2023-04-17 20:30:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Pseudomonas aeruginosa,RAW264.7 macrophages,host-microbe intraction |
Contact List
| Liang Yang | |
|---|---|
| contact affiliation | School of Medicine, Southern University of Science and Technology |
| contact email | yangl@sustech.edu.cn |
| lab head | |
| Xiangke Duan | |
| contact affiliation | Southern University of Science and Technology |
| contact email | xkeduan@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
