PXD041586

PXD041586 is an original dataset announced via ProteomeXchange.

Title	Metaproteomes of intestinal microbiomes from mice fed different sources of dietary protein
Description	Diet plays a major role in altering the composition and function of the gut microbiota. Previously most studies have focused on the effects of fiber, fat, and different amounts of protein on the gut microbiota. In this study we investigated how different sources of protein affect the gut microbiota of mice. We fed conventional and germ-free C57BL/6J mice a series of defined diets where the source of dietary protein was the key difference, which made up twenty or forty percent of the diet. The dietary protein sources used were purified protein. The diets were fed to the same mice for one week each with a fecal sample collected at the end of each week. The diets were fed in this order: standard chow, 20% soy, 20% casein, 20% rice, 40% soy, 20% yeast, 40% casein, 20% pea, 20% egg white protein, 20% chicken bone broth, and lastly at the end of the experiment half of the mice were fed the 20% soy and half the mice the 20% casein diet again as a control. We did not collect fecal samples for the chicken bone broth diet as the diet was stopped prematurely due to diet intolerance. 12 germ-free mice (6 female, 6 male) in four cages were used. 12 mice with a conventional gut microbiota in four cages were used (6 female, 6 male). One germ-free mouse was found dead after diet 5 (20% yeast) and one conventional mouse was sacrificed after the second diet (20% casein). No sample could be collected from one of the conventional mice after the 20% egg white diet.
HostingRepository	PRIDE
AnnounceDate	2024-12-19
AnnouncementXML	Submission_2024-12-18_16:51:37.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J. Alfredo Blakeley Ruiz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: mouse gut metagenome; NCBI TaxID: 410661; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Cyberlindnera jadinii; NCBI TaxID: 4903; scientific name: Oryza sativa subsp. japonica (Rice); NCBI TaxID: 39947; scientific name: Glycine max; NCBI TaxID: 3847; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Lathyrus aphaca; NCBI TaxID: 3854; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Zea mays (Maize); NCBI TaxID: 4577; scientific name: Gallus gallus gallus; NCBI TaxID: 208526;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-17 09:40:56	ID requested
⏵ 1	2024-12-18 16:51:38	announced

Blakeley-Ruiz JA, Bartlett A, McMillan AS, Awan A, Vanhoy Walsh M, Meyerhoffer AK, Vintila S, Maier JL, Richie T, Theriot CM, Kleiner M, Dietary protein source alters gut microbiota composition and function. bioRxiv, ():(2025) [pubmed]

10.1101/2024.04.04.588169;

submitter keyword: microbiome,Mouse diets, metagenomics, gut, microbiota, metaproteomics, dietary protein

Manuel Kleiner
contact affiliation	North Carolina State University
contact email	manuel_kleiner@ncsu.edu
lab head
J. Alfredo Blakeley Ruiz
contact affiliation	North Carolina State University, Raliegh, NC
contact email	jablakel@ncsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041586
     1. Label: PRIDE project
     2. Name: Metaproteomes of intestinal microbiomes from mice fed different sources of dietary protein