PXD041578

PXD041578 is an original dataset announced via ProteomeXchange.

Title	Zapnometinib treatment and influenza A virus infection induce alteration on the HLA class I ligandome of human lung adenocarcinoma cells
Description	Targeting cellular factors is considered a promising approach to combat viral infection and overcome the resistance issue associated with direct-acting antiviral (DAA) drugs administration. In the context of drug repurposing strategies, we have previously explored the antiviral efficacy of the MEK inhibitor zapnometinib against influenza virus and SARS-CoV-2. Herein, since zapnometinib was originally promoted as an anti-cancer drug, we aimed to investigate the impact of zapnometinib on the HLA class I ligandome post-treatment and IAV infection. By implementing the immunopeptidomics approach, the influence on the HLA-I allotype distribution of the HLA ligands was observed to coincide with alteration in the relative abundance of HLA-I-presented peptides. Furthermore, functional annotation analysis of the corresponding source proteins of presented ligands revealed a remarkable gene enrichment of distinct cellular processes. Taken together, zapnometinib and IAV showed a remarkable effect on the HLA-I ligandome plasticity as well as quantitative alterations in HLA ligandome composition.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:30:48.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hazem Hamza Ewess
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-17 03:50:02	ID requested
⏵ 1	2026-06-14 16:30:49	announced

10.3389/fimmu.2026.1790267;

Hamza H, Ghosh M, Rammensee HG, Planz O, Zapnometinib treatment and influenza A virus infection modulate the HLA class I ligandome in human lung adenocarcinoma cells. Front Immunol, 17():1790267(2026) [pubmed]

submitter keyword: Influenza A virus, Ligandome analysis, MEK inhibitors, Zapnometinib, MHC-I, LC-MS/MS

Prof. Dr. Oliver Planz
contact affiliation	Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
contact email	oliver.planz@uni-tuebingen.de
lab head
Hazem Hamza Ewess
contact affiliation	Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen
contact email	hazem.ewess@mnf.uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD041578

PRIDE project URI

• PRIDE
  1. PXD041578
     1. Label: PRIDE project
     2. Name: Zapnometinib treatment and influenza A virus infection induce alteration on the HLA class I ligandome of human lung adenocarcinoma cells