PXD041568

PXD041568 is an original dataset announced via ProteomeXchange.

Title	Quantitative synaptic proteomics analysis reveal OTX1 regulates complement-dependent microglia-mediated synaptic pruning in the cerebral cortex of Otx1 mutant mouse
Description	The mammalian cortex is the structural basis for learning, cognition, and movement coordination. Dysgenesis of axon dendrites and synapses in cortical neurons can hinder learning and cognitive development, leading to epilepsy. Transcription factor Otx1 plays an important role in the development of the morphology and electrophysiological activity of cortical neurons and is associated with the occurrence of epilepsy. Abnormal synaptic pruning has been proposed to be one of the molecular mechanisms underlying epilepsy. Otx1 mutant mice leads to defective axonal pruning and changes the excitability and synaptic connections of the cortical neurons. However, little is known about the molecular pathways through which the loss of Otx1 causes epilepsy. On this basis, we found that the density and morphology of dendritic spines and microglia in Otx1 mutant mice changed significantly. TMT analysis of synaptic proteins reveals that Otx1 regulates the structure and function of cortical neurons and synaptic characteristics by regulating microglia-mediated synaptic pruning through the complement system, which also has important theoretical significance and application value for effective prevention and treatment of epilepsy.
HostingRepository	iProX
AnnounceDate	2023-04-15
AnnouncementXML	Submission_2024-04-14_20:09:22.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chao Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-16 20:43:31	ID requested
⏵ 1	2024-04-14 20:09:22	announced

Dataset with its publication pending

submitter keyword: Mouse, Cerebral cortex, OTX1, Complement, TMT

Chao Wang
contact affiliation	School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University
contact email	14b922002@hit.edu.cn
lab head
Chao Wang
contact affiliation	School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University
contact email	14b922002@hit.edu.cn
dataset submitter

iProX dataset URI