PXD041561

PXD041561 is an original dataset announced via ProteomeXchange.

Title	Proteome screening of Bacillus cereus derived extracellular vesicles
Description	Extracellular vesicles (EVs) from Gram-positive bacteria have gained considerable importance as a novel transport system of virulence factors in host-pathogen interactions. Bacillus cereus is a Gram-positive human pathogen, causing gastrointestinal toxemia as well as local and systemic infections. The pathogenicity of the B. cereus has been linked to a collection of virulence factors and exotoxins. Nevertheless, the exact mechanism of virulence factor secretion and delivery to target cells is poorly understood. Here, we investigate the production and characterization of enterotoxin-associated EVs from the enteropathogenic B. cereus strain NVH0075-95 by using a proteomics approach and studied their interaction with human host cells in vitro. Proteomic analysis of B. cereus EVs revealed virulence-associated factors, such as sphingomyelinase, phospholipase C, and the three-component enterotoxin Nhe. The detection of Nhe subunits was confirmed by immunoblotting, showing that the low abundant subunit NheC was exclusively detected in EVs as compared to vesicle-free supernatant. Fusion of B. cereus EVs with the plasma membrane of intestinal epithelial Caco2 cells represent entry routes for delivery of Nhe components to host cells, which was assessed by confocal microscopy. B. cereus EVs also elicit an inflammatory response in human monocytes and contribute to erythrocyte lysis via a cooperative interaction of enterotoxin Nhe and sphingomyelinase. Our results provide insights into the interaction of EVs from B. cereus with human host cells and add a new layer of complexity to our understanding of multicomponent enterotoxin assembly, offering new opportunities to decipher molecular processes involved in disease development.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:36.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Buchacher
SpeciesList	scientific name: Bacillus cereus; NCBI TaxID: 1396;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-16 01:20:50	ID requested
1	2023-07-20 11:53:44	announced
⏵ 2	2023-11-14 09:03:39	announced	2023-11-14: Updated project metadata.

Buchacher T, Digruber A, Kranzler M, Del Favero G, Ehling-Schulz M, Bacillus cereus extracellular vesicles act as shuttles for biologically active multicomponent enterotoxins. Cell Commun Signal, 21(1):112(2023) [pubmed]

submitter keyword: proteomics,Bacillus cereus, extracellular vesicles

Dr Monika Ehling-Schulz
contact affiliation	Functional Microbiology, Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria
contact email	monika.ehling-schulz@vetmeduni.ac.at
lab head
Tanja Buchacher
contact affiliation	Turku Bioscience Finland
contact email	tanja.buchacher@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041561

PRIDE project URI

• PRIDE
  1. PXD041561
     1. Label: PRIDE project
     2. Name: Proteome screening of Bacillus cereus derived extracellular vesicles