PXD041556

PXD041556 is an original dataset announced via ProteomeXchange.

Title	Human Cell 293T BCAT1 IP GC-LC-MS
Description	To identify the potential interaction protein that interacts with BCAT1 by IP-MS, we established the stable cell line with vector and BCAT1 in AGS. Then ten million cells were collected lysed in ice-cold 0.1% NP-40 buffer (50 mM Na2HPO4, pH 7.5, 150 mM NaCl) with protease inhibitors cocktail (Sigma, p8340). The supernatants were collected by centrifugation and incubated with 10 µL Anti Flag‐beads (Sigma, A2220at 4°C for overnight. After incubation, beads were washed with lysis buffer for 3 times. Precipitates were resuspended in 1 × SDS loading buffer and separated by SDS-PAGE (polyacrylamide gel electrophoresis). Then cut off (about 1cm2) gel for trypsin hydrolysis and collected and dried the peptide for MS analysis (Thermo Fisher Q Exactive).
HostingRepository	PRIDE
AnnounceDate	2024-11-15
AnnouncementXML	Submission_2024-11-15_00:06:42.245.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaochen Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-15 05:57:07	ID requested
⏵ 1	2024-11-15 00:06:43	announced

10.1038/s42255-023-00818-7;

Qian L, Li N, Lu XC, Xu M, Liu Y, Li K, Zhang Y, Hu K, Qi YT, Yao J, Wu YL, Wen W, Huang S, Chen ZJ, Yin M, Lei QY, Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression. Nat Metab, 5(7):1159-1173(2023) [pubmed]

submitter keyword: Huamn BCAT1 IP-MS

Lei Qun-Ying
contact affiliation	Fudan University
contact email	lxc568377@163.com
lab head
Xiaochen Lu
contact affiliation	Fudan University
contact email	lxc568377@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD041556

PRIDE project URI

• PRIDE
  1. PXD041556
     1. Label: PRIDE project
     2. Name: Human Cell 293T BCAT1 IP GC-LC-MS