PXD041549

PXD041549 is an original dataset announced via ProteomeXchange.

Title	ADAR1-mediated RNA editing promotes B cell lymphomagenesis.
Description	The project aims to evaluate the contribution of ADAR1 RNA editing to B cell lymphomagenesis, specifically in diffuse large B cell lymphoma (DLBCL). Within our DLBCL cohort, RNA editing targets transcripts within known lymphoma-driving pathways such as apoptosis, p53 and NF-kB signaling, as well as the previously unrecognized RIG-I-like pathway. In the latter context, we show that ADAR1-mediated editing in the MAVS transcript correlates with increased MAVS protein expression levels, associating with increased interferon/NF-kB signaling and increased T cell exhaustion. To confirm this mechanism, we have performed LC-MSMS analysis on a DLBCL cell line (RCK8) in the presence or absence of ADAR1 (Figure S11D ). Additionally, using targeted RNA base editing tools to restore editing within MAVS 3'UTR in ADAR1-deficient cells, we demonstrate that editing is likely to be causal to an increase in downstream signaling in the absence of activation by canonical nucleic acid receptor sensing. To confirm that this signaling increase depends on an increase of MAVS protein upon specific editing, we have performed LC-MSMS analysis on the same samples (Figure 4G).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:54.476.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Pecori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-14 10:02:21	ID requested
1	2023-07-20 12:37:54	announced
⏵ 2	2023-11-14 09:04:00	announced	2023-11-14: Updated project metadata.

Pecori R, Ren W, Pirmoradian M, Wang X, Liu D, Berglund M, Li W, Tasakis RN, Di Giorgio S, Ye X, Li X, Arnold A, W, ü, st S, Schneider M, Selvasaravanan KD, Fuell Y, Stafforst T, Amini RM, Sonnevi K, Enblad G, Sander B, Wahlin BE, Wu K, Zhang H, Helm D, Binder M, Papavasiliou FN, Pan-Hammarstr, ö, m Q, ADAR1-mediated RNA editing promotes B cell lymphomagenesis. iScience, 26(6):106864(2023) [pubmed]

submitter keyword: ADAR1, MAVS,RCK8, DLBCL

Nina Fotini Papavasiliou
contact affiliation	Division of Immune Diversity (D150), German Cancer Research Center (DKFZ), Heidelberg, Germany.
contact email	n.papavasiliou@dkfz-heidelberg.de
lab head
Riccardo Pecori
contact affiliation	German Cancer Research Center (DKFZ)
contact email	r.pecori@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD041549

PRIDE project URI

• PRIDE
  1. PXD041549
     1. Label: PRIDE project
     2. Name: ADAR1-mediated RNA editing promotes B cell lymphomagenesis.