PXD041547

PXD041547 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of exosomes derived from endothelial cells and Schwann cells
Description	Exosomes derived from endothelial cells and Schwann cells have been employed as novel treatments of neurological diseases, including peripheral neuropathy. Exosomal cargo plays a critical role in mediating recipient cell function. In this study, we thus performed a comprehensive proteomic analysis of exosomes derived from healthy mouse dermal microvascular endothelial cells (EC-Exo) and healthy mouse Schwann cells (SC-Exo). We detected 1,817and 1,579 proteins in EC-Exo and SC-Exo, respectively. Among them, 1506 proteins were present in both EC-Exo and SC-Exo, while 311 and 73 proteins were detected only in EC-Exo and SC-Exo, respectively. Bioinformatic analysis revealed that EC-Exo enriched proteins were involved in the neurovascular function, while SC-Exo enriched proteins were related to lipid metabolism. Western blot analysis of 14 enriched proteins revealed that EC-Exo contained proteins involved in mediating endothelial function such as delta-like 4 (DLL4) and endothelial NOS (NOS3), whereas SC-Exo had proteins involved in mediating glial function such as apolipoprotein A-I (APOA1) and phospholipid transfer protein (PLTP). Collectively, the present study indicates cargo protein profiles are distinct between EC-Exo and SC-Exo, suggesting potential roles of EC-Exo and SC-Exo mainly in mediating neurovascular and myelin functions, respectively.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:49.634.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041547
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Stemmer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-14 08:50:01	ID requested
1	2023-08-20 01:47:13	announced
2	2023-11-14 09:04:46	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:00:50	announced	2024-10-22: Updated project metadata.

Wang L, Lu X, Chopp M, Li C, Zhang Y, Szalad A, Liu XS, Zhang ZG, Comparative proteomic analysis of exosomes derived from endothelial cells and Schwann cells. PLoS One, 18(8):e0290155(2023) [pubmed]

10.6019/PXD041547;

10.1371/journal.pone.0290155;

submitter keyword: proteomics,exosomes, endothelial cells, protein, Schwann cells

Lei Wang
contact affiliation	Department of Neurology, Henry Ford Health
contact email	lwang1@hfhs.org
lab head
Paul Stemmer
contact affiliation	Wayne State University
contact email	pmstemmer@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD041547
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of exosomes derived from endothelial cells and Schwann cells