PXD041544

PXD041544 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of expanded resting and activated human MAIT (mucosal associated invariant T cells)
Description	Mucosal associated invariant T (MAIT) cells are an abundant population of innate T cells that recognize bacterial ligands and play a key role in host protection against bacterial and viral pathogens. Upon activation, MAIT cells undergo proliferative expansion and increase their production of effector molecules such as cytokines. In this study, we found that mRNA and protein the abundance of the key metabolism regulator and transcription factor MYC was increased in stimulated MAIT cells. Using quantitative mass spectrometry, we identified the activation of two MYC controlled metabolic pathways, amino acid transport and glycolysis, both of which were necessary for MAIT cell proliferation. Finally, we showed that MAIT cells isolated from people with obesity showed decreased MYC mRNA abundance upon activation, which was associated with defective MAIT cell proliferation and functional responses. Collectively, our data uncovers the importance of MYC-regulated metabolism for MAIT cell proliferation and provides additional insight into the molecular basis for the functional defects of MAIT cells in obesity.
HostingRepository	PRIDE
AnnounceDate	2023-04-20
AnnouncementXML	Submission_2023-04-20_03:09:22.584.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndrewHowden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-14 07:45:01	ID requested
⏵ 1	2023-04-20 03:09:22	announced

Dataset with its publication pending

submitter keyword: T-lymphoctes, T-cells, Myc, proteomics,MAIT, human

Dr AndrewHogan
contact affiliation	Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co Kildare. Ireland.
contact email	andrew.e.hogan@mu.ie
lab head
AndrewHowden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD041544

PRIDE project URI

• PRIDE
  1. PXD041544
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of expanded resting and activated human MAIT (mucosal associated invariant T cells)