PXD041531

PXD041531 is an original dataset announced via ProteomeXchange.

Title	Prolyl 4-hydroxylase alpha-2 directly activates mTOR kinase
Description	To identify candidate mTOR-interacting molecules, we performed an anti-Flag immunoprecipitation (IP) followed by mass spectrometry (MS)-based proteomic analysis in 293T cells overexpressing Flag-tagged mTOR. We identified P4HA2 binding proteins with anti-HA IP followed by (MS)-based proteomic analysis in 293T cells overexpressing HA-tagged P4HA2. To speculate that mTOR can be hydroxylated by P4HA2. We used 293T cells overexpressing Flag-mTOR, Flag-mTOR/HA-P4HA2, and Flag-mTOR/HA-P4HA2-overexpressing cells treated with EDHB, we conducted an anti-Flag IP to capture and enrich mTOR protein, and the immunoprecipitates were subjected to MS analysis.
HostingRepository	iProX
AnnounceDate	2023-04-14
AnnouncementXML	Submission_2023-04-13_21:29:55.053.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ersuo Jin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-13 21:29:26	ID requested
⏵ 1	2023-04-13 21:29:55	announced

Dataset with its publication pending

submitter keyword: P4HA2, mTOR, HEK293, MS, hydroxylation

Hongtao Zhang
contact affiliation	Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University; Department of Genetics, School of Biology and Basic Medical Sciences, Medical College of Soochow University
contact email	htzhang@suda.edu.cn
lab head
Ersuo Jin
contact affiliation	Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University; Department of Genetics, School of Biology and Basic Medical Sciences, Medical College of Soochow University
contact email	719138260@qq.com
dataset submitter

iProX dataset URI