PXD041531
PXD041531 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Prolyl 4-hydroxylase alpha-2 directly activates mTOR kinase |
| Description | To identify candidate mTOR-interacting molecules, we performed an anti-Flag immunoprecipitation (IP) followed by mass spectrometry (MS)-based proteomic analysis in 293T cells overexpressing Flag-tagged mTOR. We identified P4HA2 binding proteins with anti-HA IP followed by (MS)-based proteomic analysis in 293T cells overexpressing HA-tagged P4HA2. To speculate that mTOR can be hydroxylated by P4HA2. We used 293T cells overexpressing Flag-mTOR, Flag-mTOR/HA-P4HA2, and Flag-mTOR/HA-P4HA2-overexpressing cells treated with EDHB, we conducted an anti-Flag IP to capture and enrich mTOR protein, and the immunoprecipitates were subjected to MS analysis. |
| HostingRepository | iProX |
| AnnounceDate | 2023-04-14 |
| AnnouncementXML | Submission_2024-06-06_00:47:03.621.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ersuo Jin |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue |
| Instrument | LTQ Orbitrap Elite; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-04-13 21:29:26 | ID requested | |
| 1 | 2023-04-13 21:29:55 | announced | |
| ⏵ 2 | 2024-06-06 00:47:04 | announced | 2024-06-06: Update information. |
Publication List
| Jin E, Wang S, Chen D, Wang JP, Zeng Y, Sun R, Zhang HT, P4HA2 activates mTOR via hydroxylation and targeting P4HA2-mTOR inhibits lung adenocarcinoma cell growth. Oncogene, 43(24):1813-1823(2024) [pubmed] |
Keyword List
| submitter keyword: P4HA2, mTOR, HEK293, MS, hydroxylation |
Contact List
| Hongtao Zhang | |
|---|---|
| contact affiliation | Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University; Department of Genetics, School of Biology and Basic Medical Sciences, Medical College of Soochow University |
| contact email | htzhang@suda.edu.cn |
| lab head | |
| Ersuo Jin | |
| contact affiliation | Soochow University Laboratory of Cancer Molecular Genetics, Medical College of Soochow University; Department of Genetics, School of Biology and Basic Medical Sciences, Medical College of Soochow University |
| contact email | 719138260@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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