PXD041524

PXD041524 is an original dataset announced via ProteomeXchange.

Title	A rapid and efficient method for the extraction of histone proteins
Description	Current protocols used to extract and purify histones are notoriously tedious, especially when using yeast cells. Here, we describe the use of a simple filter-aided sample preparation approach enabling histone extraction from yeast and mammalian cells using acidified ethanol, a condition that not only improves extraction but also inactivates histone-modifying enzymes. We show that our improved method preserves the integrity of histones, and prevents N-terminal clipping of H3, an artefact frequently observed in yeast cells using standard histone extraction protocols. Our method is scalable and provides efficient recovery of histones when extracts are prepared from as little as two million yeast cells. We further demonstrate the application of this approach for the analysis of histone modifications including fungal clinical isolates available in limited quantity. Altogether, this method enables the study of histones and their modifications in a faster, simpler, and more robust manner.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:00:05.980.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD041524
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eric Bonneil
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monomethylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-13 11:29:11	ID requested
1	2023-08-10 06:34:59	announced
2	2023-11-14 09:04:33	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:00:07	announced	2024-10-22: Updated project metadata.

10.6019/PXD041524;

10.1021/acs.jproteome.3c00266;

Homsi C, Rajan RE, Minati R, St-Hilaire E, Bonneil E, Dufresne SF, Wurtele H, Verreault A, Thibault P, A Rapid and Efficient Method for the Extraction of Histone Proteins. J Proteome Res, 22(8):2765-2773(2023) [pubmed]

submitter keyword: N-terminal clipping,Histone extratction, FASP

Pierre Thibault
contact affiliation	IRIC-Universite de Montreal
contact email	pierre.thibault@umontreal.ca
lab head
Eric Bonneil
contact affiliation	Proteomic Platform
contact email	eric.bonneil@umontreal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD041524

PRIDE project URI

• PRIDE
  1. PXD041524
     1. Label: PRIDE project
     2. Name: A rapid and efficient method for the extraction of histone proteins