PXD041522

PXD041522 is an original dataset announced via ProteomeXchange.

Title	Dynamic N-glycan rearrangement nucleates galectin-3 oligomers and drives endocytosis
Description	Membrane glycoproteins often adopt different conformations when shifting between active and inactive states. Here, we discover a molecular switch that operates through major changes in the spatial arrangement of the N-glycan landscape during such conformational transitions. We demonstrate that the cell adhesion glycoprotein alpha5 beta1 integrin selectively in its bent-closed inactive conformational state nucleates the formation of ring-shaped tetramers of the glycan-binding protein galectin-3 to drive endocytic uptake. We propose a novel structural model to explain how spatial rearrangement of N-glycans transduced by conformational changes results in galectin-3 tetramers. Our findings identify local arrangements of N-glycans as unexpectedly dynamic regulatory elements at the cell surface and provide a new facet to higher order pattern interactions with glycoproteins for regulation of processes such as galectin-dependent endocytosis.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:40:49.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agnès Hovasse
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	complex glycosylation
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-04-13 08:15:39	ID requested
⏵ 1	2025-11-09 16:40:50	announced

Shafaq-Zadah M, Dransart E, Hamitouche I, Wunder C, Chambon V, Valades-Cruz CA, Leconte L, Sarangi NK, Robinson J, Bai SK, Regmi R, Di Cicco A, Hovasse A, Bartels R, Nilsson UJ, Cianf, é, rani-Sanglier S, Leffler H, Keyes TE, L, é, vy D, Raunser S, Roderer D, Johannes L, integrin nucleates galectin-3 oligomers to determine endocytic fate. Nat Commun, 16(1):9461(2025) [pubmed]

10.1038/s41467-025-64523-7;

submitter keyword: Cross-Link,Glycoprotein, Structural

Agnes Hovasse
contact affiliation	CNRS-IPHC-LSMBO
contact email	ahovasse@unistra.fr
lab head
Agnès Hovasse
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique, University of Strasbourg, CNRS, Institut Pluridisciplinaire Hubert Curien, UMR 7178
contact email	ahovasse@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD041522

PRIDE project URI

• PRIDE
  1. PXD041522
     1. Label: PRIDE project
     2. Name: Dynamic N-glycan rearrangement nucleates galectin-3 oligomers and drives endocytosis